Detailed information of TA system TA243925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1385564..1386252
• Replicon: chromosome
• Accession: NZ_CP096846
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2377

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: M1775_RS06630
• Protein ID: WP_003406304.1
• Coordinates: 1385821..1386252 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: M1775_RS06625
• Protein ID: WP_003406302.1
• Coordinates: 1385564..1385824 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1775_RS06605 (M1775_06600)	1381141..1381965	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
M1775_RS06610 (M1775_06605)	1381970..1383151	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
M1775_RS06615 (M1775_06610)	1383228..1384328	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
M1775_RS06620 (M1775_06615)	1384499..1385488	+	990	WP_003406301.1	malate dehydrogenase	-
M1775_RS06625 (M1775_06620)	1385564..1385824	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
M1775_RS06630 (M1775_06625)	1385821..1386252	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1775_RS06635 (M1775_06630)	1386275..1387961	-	1687	Protein_1309	PE family protein	-
M1775_RS06640 (M1775_06635)	1388141..1389001	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
M1775_RS06645 (M1775_06640)	1389082..1389912	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
M1775_RS06650 (M1775_06645)	1389969..1390262	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
M1775_RS06655 (M1775_06650)	1390259..1390528	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T243925 WP_003406304.1 NZ_CP096846:1385821-1386252 [Mycobacterium tuberculosis variant bovis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T243925 UFRD01000002:4453607-4454005 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT243925 WP_003406302.1 NZ_CP096846:1385564-1385824 [Mycobacterium tuberculosis variant bovis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT243925 UFRD01000002:4453380-4453607 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6