Detailed information of TA system TA243924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240820..1241388
• Replicon: chromosome
• Accession: NZ_CP096846
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2377

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: M1775_RS05955
• Protein ID: WP_003405865.1
• Coordinates: 1241014..1241388 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: M1775_RS05950
• Protein ID: WP_003405863.1
• Coordinates: 1240820..1241017 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1775_RS05930 (M1775_05930)	1236861..1237499	-	639	WP_011799158.1	lipid droplet-associated protein	-
M1775_RS05935 (M1775_05935)	1237589..1238596	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
M1775_RS05940 (M1775_05940)	1238613..1239596	-	984	WP_003898731.1	hypothetical protein	-
M1775_RS05945 (M1775_05945)	1239659..1240732	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
M1775_RS05950 (M1775_05950)	1240820..1241017	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1775_RS05955 (M1775_05955)	1241014..1241388	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1775_RS05960 (M1775_05960)	1241591..1242289	+	699	WP_010950494.1	hypothetical protein	-
M1775_RS05965 (M1775_05965)	1242407..1242592	+	186	WP_003901093.1	hypothetical protein	-
M1775_RS05970 (M1775_05970)	1242519..1242794	-	276	WP_003405867.1	hypothetical protein	-
M1775_RS05975 (M1775_05975)	1243037..1243360	+	324	WP_003405871.1	putative quinol monooxygenase	-
M1775_RS05980 (M1775_05980)	1243375..1244073	-	699	WP_031646953.1	hypothetical protein	-
M1775_RS05985 (M1775_05985)	1244107..1244316	+	210	WP_003911400.1	hypothetical protein	-
M1775_RS05990 (M1775_05990)	1244268..1245038	-	771	Protein_1180	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T243924 WP_003405865.1 NZ_CP096846:1241014-1241388 [Mycobacterium tuberculosis variant bovis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T243924 UFRD01000002:4442740-4443153 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT243924 WP_003405863.1 NZ_CP096846:1240820-1241017 [Mycobacterium tuberculosis variant bovis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT243924 UFRD01000002:4442493-4442759 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07