Detailed information of TA system TA243917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 756316..757108
• Replicon: chromosome
• Accession: NZ_CP096846
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2377

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: M1775_RS03505
• Protein ID: WP_003403386.1
• Coordinates: 756671..757108 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: M1775_RS03500
• Protein ID: WP_003403381.1
• Coordinates: 756316..756561 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1775_RS03470 (M1775_03470)	751336..752841	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
M1775_RS03475 (M1775_03475)	752922..753932	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
M1775_RS03480 (M1775_03480)	754320..754703	-	384	WP_003403365.1	ribonuclease VapC6	-
M1775_RS03485 (M1775_03485)	754798..754953	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
M1775_RS03490 (M1775_03490)	755029..755745	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
M1775_RS03495 (M1775_03495)	756021..756329	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
M1775_RS03500 (M1775_03500)	756316..756561	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
M1775_RS03505 (M1775_03505)	756671..757108	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1775_RS03510 (M1775_03510)	757105..757359	-	255	WP_003911263.1	antitoxin VapB7	-
M1775_RS03515 (M1775_03515)	757473..759836	+	2364	WP_003403397.1	arylsulfatase AtsD	-
M1775_RS03520 (M1775_03520)	759899..760225	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
M1775_RS03525 (M1775_03525)	760137..760475	+	339	WP_003403405.1	PIN domain-containing protein	-
M1775_RS03530 (M1775_03530)	760472..760645	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T243917 WP_003403386.1 NZ_CP096846:c757108-756671 [Mycobacterium tuberculosis variant bovis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T243917 UFRD01000002:c2963869-2963585 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT243917 WP_003403381.1 NZ_CP096846:c756561-756316 [Mycobacterium tuberculosis variant bovis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT243917 UFRD01000002:c2964101-2963859 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0