Detailed information of TA system TA243911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 704056..704699
• Replicon: chromosome
• Accession: NZ_CP096846
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2377

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: M1775_RS03210
• Protein ID: WP_003403187.1
• Coordinates: 704298..704699 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: M1775_RS03205
• Protein ID: WP_003403184.1
• Coordinates: 704056..704301 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1775_RS03170 (M1775_03170)	699336..699866	-	531	WP_003917320.1	two component system sensor kinase HK2	-
M1775_RS03175 (M1775_03175)	699850..700545	-	696	WP_197044064.1	two-component system response regulator TcrA	-
M1775_RS03180 (M1775_03180)	700668..700979	+	312	WP_003403164.1	hypothetical protein	-
M1775_RS03185 (M1775_03185)	701051..702001	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
M1775_RS03190 (M1775_03190)	702242..702826	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
M1775_RS03195 (M1775_03195)	702828..703538	+	711	Protein_633	IS607 family element RNA-guided endonuclease TnpB	-
M1775_RS03200 (M1775_03200)	703541..704011	+	471	WP_003898523.1	hypothetical protein	-
M1775_RS03205 (M1775_03205)	704056..704301	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1775_RS03210 (M1775_03210)	704298..704699	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1775_RS03215 (M1775_03215)	704690..704869	+	180	Protein_637	hypothetical protein	-
M1775_RS03220 (M1775_03220)	705287..705484	-	198	WP_003403191.1	hypothetical protein	-
M1775_RS03225 (M1775_03225)	705564..706721	-	1158	WP_003403193.1	hypothetical protein	-
M1775_RS03230 (M1775_03230)	706773..706994	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
M1775_RS03235 (M1775_03235)	707136..707741	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T243911 WP_003403187.1 NZ_CP096846:704298-704699 [Mycobacterium tuberculosis variant bovis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T243911 NZ_OY019090:2060-2335 [Escherichia coli O25b:H4-ST131]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT243911 WP_003403184.1 NZ_CP096846:704056-704301 [Mycobacterium tuberculosis variant bovis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT243911 NZ_OY019090:1776-2060 [Escherichia coli O25b:H4-ST131]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCTCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91