Detailed information of TA system TA243910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697966..698612
• Replicon: chromosome
• Accession: NZ_CP096846
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2377

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: M1775_RS03155
• Protein ID: WP_003403137.1
• Coordinates: 697966..698379 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: M1775_RS03160
• Protein ID: WP_003403139.1
• Coordinates: 698376..698612 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1775_RS03135 (M1775_03135)	694049..695599	+	1551	WP_003403119.1	MlaD family protein	-
M1775_RS03140 (M1775_03140)	695651..696043	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
M1775_RS03145 (M1775_03145)	696040..696297	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
M1775_RS03150 (M1775_03150)	696480..697715	-	1236	WP_003403128.1	ATP-binding protein	-
M1775_RS03155 (M1775_03155)	697966..698379	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1775_RS03160 (M1775_03160)	698376..698612	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
M1775_RS03165 (M1775_03165)	698716..699222	-	507	WP_003900973.1	two component system sensor kinase HK1	-
M1775_RS03170 (M1775_03170)	699336..699866	-	531	WP_003917320.1	two component system sensor kinase HK2	-
M1775_RS03175 (M1775_03175)	699850..700545	-	696	WP_197044064.1	two-component system response regulator TcrA	-
M1775_RS03180 (M1775_03180)	700668..700979	+	312	WP_003403164.1	hypothetical protein	-
M1775_RS03185 (M1775_03185)	701051..702001	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
M1775_RS03190 (M1775_03190)	702242..702826	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
M1775_RS03195 (M1775_03195)	702828..703538	+	711	Protein_633	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T243910 WP_003403137.1 NZ_CP096846:c698379-697966 [Mycobacterium tuberculosis variant bovis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T243910 NZ_OY019089:157361-157777 [Escherichia coli O25b:H4-ST131]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT243910 WP_003403139.1 NZ_CP096846:c698612-698376 [Mycobacterium tuberculosis variant bovis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT243910 NZ_OY019089:157134-157364 [Escherichia coli O25b:H4-ST131]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89