Detailed information of TA system TA243905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 364427..364998
• Replicon: chromosome
• Accession: NZ_CP096846
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2377

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: M1775_RS01590
• Protein ID: WP_003401560.1
• Coordinates: 364427..364729 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: M1775_RS01595
• Protein ID: WP_003401563.1
• Coordinates: 364777..364998 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1775_RS01570 (M1775_01570)	359851..360654	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
M1775_RS01575 (M1775_01575)	360664..362061	-	1398	WP_003401544.1	sulfatase	-
M1775_RS01580 (M1775_01580)	362240..364060	+	1821	WP_003401546.1	PE family protein	-
M1775_RS01585 (M1775_01585)	364203..364430	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
M1775_RS01590 (M1775_01590)	364427..364729	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
M1775_RS01595 (M1775_01595)	364777..364998	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
M1775_RS01600 (M1775_01600)	364995..365420	+	426	WP_003401566.1	PIN domain nuclease	-
M1775_RS01605 (M1775_01605)	365556..366188	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
M1775_RS01610 (M1775_01610)	366185..367093	+	909	WP_003900117.1	protochlorophyllide reductase	-
M1775_RS01615 (M1775_01615)	367101..367691	-	591	WP_234712434.1	hypothetical protein	-
M1775_RS01620 (M1775_01620)	367684..367734	-	51	Protein_320	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T243905 WP_003401560.1 NZ_CP096846:364427-364729 [Mycobacterium tuberculosis variant bovis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T243905 NZ_OY019088:c4207741-4207523 [Escherichia coli O25b:H4-ST131]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT243905 WP_003401563.1 NZ_CP096846:364777-364998 [Mycobacterium tuberculosis variant bovis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT243905 NZ_OY019088:c4208141-4207767 [Escherichia coli O25b:H4-ST131]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48