Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2827388..2828019 Replicon chromosome
Accession NZ_CP096845
Organism Mycobacterium tuberculosis variant bovis strain Mb1101

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag M1777_RS13325 Protein ID WP_003413174.1
Coordinates 2827642..2828019 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag M1777_RS13320 Protein ID WP_003413167.1
Coordinates 2827388..2827645 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1777_RS13285 (M1777_13295) 2822646..2822960 + 315 WP_009937839.1 hypothetical protein -
M1777_RS13290 (M1777_13300) 2823256..2824467 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
M1777_RS13295 (M1777_13305) 2824594..2825253 + 660 WP_003900846.1 LppA family lipoprotein -
M1777_RS13300 (M1777_13310) 2825250..2825909 + 660 WP_003900847.1 LppA family lipoprotein -
M1777_RS13305 (M1777_13315) 2825906..2826568 + 663 WP_003900848.1 LppA family lipoprotein -
M1777_RS13310 (M1777_13320) 2826565..2826843 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
M1777_RS13315 (M1777_13325) 2826936..2827349 + 414 WP_003413164.1 PIN domain nuclease -
M1777_RS13320 (M1777_13330) 2827388..2827645 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
M1777_RS13325 (M1777_13335) 2827642..2828019 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
M1777_RS13330 (M1777_13340) 2828035..2828409 - 375 WP_003413177.1 hypothetical protein -
M1777_RS13335 (M1777_13345) 2828509..2828904 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
M1777_RS13340 (M1777_13350) 2828901..2829146 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
M1777_RS13345 (M1777_13355) 2829557..2829976 - 420 WP_273585834.1 A24 family peptidase -
M1777_RS13350 (M1777_13360) 2829988..2830796 - 809 Protein_2635 shikimate dehydrogenase -
M1777_RS13355 (M1777_13365) 2830793..2832046 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
M1777_RS13360 (M1777_13370) 2832039..2832551 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T243889 WP_003413174.1 NZ_CP096845:2827642-2828019 [Mycobacterium tuberculosis variant bovis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T243889 NZ_OY019088:c831982-831728 [Escherichia coli O25b:H4-ST131]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT243889 WP_003413167.1 NZ_CP096845:2827388-2827645 [Mycobacterium tuberculosis variant bovis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT243889 NZ_OY019088:c832230-831979 [Escherichia coli O25b:H4-ST131]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References