Detailed information of TA system TA243878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2199828..2200516
• Replicon: chromosome
• Accession: NZ_CP096845
• Organism: Mycobacterium tuberculosis variant bovis strain Mb1101

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0A653
• Locus tag: M1777_RS10325
• Protein ID: WP_003409958.1
• Coordinates: 2199828..2200247 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: M1777_RS10330
• Protein ID: WP_003409968.1
• Coordinates: 2200256..2200516 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1777_RS10295 (M1777_10305)	2194834..2195139	+	306	Protein_2034	ABC transporter permease	-
M1777_RS10300 (M1777_10310)	2195135..2195215	+	81	Protein_2035	hypothetical protein	-
M1777_RS10305 (M1777_10315)	2195323..2196171	+	849	WP_273585820.1	class I SAM-dependent methyltransferase	-
M1777_RS10310 (M1777_10320)	2196134..2197579	-	1446	WP_003409946.1	APC family permease	-
M1777_RS10315 (M1777_10325)	2197758..2198444	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
M1777_RS10320 (M1777_10330)	2198635..2199603	-	969	WP_003409956.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
M1777_RS10325 (M1777_10335)	2199828..2200247	-	420	WP_003409958.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1777_RS10330 (M1777_10340)	2200256..2200516	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1777_RS10335 (M1777_10345)	2200659..2202334	+	1676	Protein_2042	PecA family PE domain-processing aspartic protease	-
M1777_RS10340 (M1777_10350)	2202322..2202975	-	654	WP_003409976.1	cutinase Cfp21	-
M1777_RS10345 (M1777_10355)	2203090..2203320	+	231	WP_003409980.1	DUF167 domain-containing protein	-
M1777_RS10350 (M1777_10360)	2203405..2204316	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
M1777_RS10355 (M1777_10365)	2204425..2205024	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T243878 WP_003409958.1 NZ_CP096845:c2200247-2199828 [Mycobacterium tuberculosis variant bovis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T243878 NZ_OX638701:c1428838-1428557 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT243878 WP_003409968.1 NZ_CP096845:c2200516-2200256 [Mycobacterium tuberculosis variant bovis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT243878 NZ_OX638701:c1429062-1428835 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: A0A829C4H9

Antitoxin

• AlphaFold DB: A0A829C483