Detailed information of TA system TA243875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2181777..2182480
• Replicon: chromosome
• Accession: NZ_CP096845
• Organism: Mycobacterium tuberculosis variant bovis strain Mb1101

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: M1777_RS10175
• Protein ID: WP_003409778.1
• Coordinates: 2181777..2182106 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: M1777_RS10180
• Protein ID: WP_003409780.1
• Coordinates: 2182103..2182480 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1777_RS10155 (M1777_10165)	2178160..2179230	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
M1777_RS10160 (M1777_10170)	2179227..2179742	+	516	WP_003409718.1	flavin reductase family protein	-
M1777_RS10165 (M1777_10175)	2179739..2180800	+	1062	WP_273585819.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
M1777_RS10170 (M1777_10180)	2180797..2181567	+	771	WP_003409775.1	SDR family oxidoreductase	-
M1777_RS10175 (M1777_10185)	2181777..2182106	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1777_RS10180 (M1777_10190)	2182103..2182480	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
M1777_RS10185 (M1777_10195)	2182477..2183067	-	591	WP_003910477.1	SEC-C metal-binding domain-containing protein	-
M1777_RS10190 (M1777_10200)	2183122..2184486	+	1365	WP_003899094.1	HNH endonuclease signature motif containing protein	-
M1777_RS10195 (M1777_10205)	2184641..2185093	-	453	WP_010950636.1	lipoprotein	-
M1777_RS10200 (M1777_10210)	2185157..2185558	+	402	WP_003409869.1	hypothetical protein	-
M1777_RS10205 (M1777_10215)	2185551..2185733	-	183	WP_003409870.1	hypothetical protein	-
M1777_RS10210 (M1777_10220)	2185847..2186197	-	351	WP_003409871.1	hypothetical protein	-
M1777_RS10215 (M1777_10225)	2186208..2187110	-	903	WP_003409874.1	hypothetical protein	-
M1777_RS10220 (M1777_10230)	2187131..2187322	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T243875 WP_003409778.1 NZ_CP096845:c2182106-2181777 [Mycobacterium tuberculosis variant bovis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T243875 NZ_OX638612:33014-33334 [Pseudomonas aeruginosa]
ATGCAGCGAGGCGACCTCGTGACGGTTTCATTGCAGGGTGACTACGGCAAACCACGACCAGCCCTGATCGTTCAATCAGA
CCTACTAACCGACTTGGAAAGCGTTGTGCTGTGCCCAGTGACAAGCGACCTGCGCAATGCCGCGTTTCGCGTCACCGTGG
AACCGAATCCAGCTAACGGGCTGCGCGCACTATCGCAAGTTATGGTGGACAAGCTCTCGACGCTACCGCGCACCAAAATC
AGTGAACCGTTTGGCCGCCTCGACGATGAGCGAATGAAAGCAATAGATAGGGCGCTCCTGTTGGTCGTTGGCGTTATTTG
A

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT243875 WP_003409780.1 NZ_CP096845:c2182480-2182103 [Mycobacterium tuberculosis variant bovis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT243875 NZ_OX638612:32799-33014 [Pseudomonas aeruginosa]
ATGCCATCAATTCACGAATCAAAGGTAGCCCGACACCGCGAACGGATGCGCGCGGCGGGGCTGCGGCCTGTGCAGTTTTG
GGTGCCGGACACCCGTTCGCCTGAGTTCACGGCGCAAGTGCGCAAGCAATGCCAAAGCCTGAAGGGCGACCCAGCAGAAG
CAGAGGTTTTGCGCTTCACCGAAGAGGCGGCATCGCACGTTGAGGGCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8