Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 753249..754041 Replicon chromosome
Accession NZ_CP096845
Organism Mycobacterium tuberculosis variant bovis strain Mb1101

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag M1777_RS03500 Protein ID WP_003403386.1
Coordinates 753604..754041 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag M1777_RS03495 Protein ID WP_003403381.1
Coordinates 753249..753494 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1777_RS03465 (M1777_03465) 748269..749774 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
M1777_RS03470 (M1777_03470) 749855..750865 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
M1777_RS03475 (M1777_03475) 751253..751636 - 384 WP_003403365.1 ribonuclease VapC6 -
M1777_RS03480 (M1777_03480) 751731..751886 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
M1777_RS03485 (M1777_03485) 751962..752678 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
M1777_RS03490 (M1777_03490) 752954..753262 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
M1777_RS03495 (M1777_03495) 753249..753494 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
M1777_RS03500 (M1777_03500) 753604..754041 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
M1777_RS03505 (M1777_03505) 754038..754292 - 255 WP_003911263.1 antitoxin VapB7 -
M1777_RS03510 (M1777_03510) 754406..756769 + 2364 WP_003403397.1 arylsulfatase AtsD -
M1777_RS03515 (M1777_03515) 756832..757158 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M1777_RS03520 (M1777_03520) 757070..757408 + 339 WP_003403405.1 PIN domain-containing protein -
M1777_RS03525 (M1777_03525) 757405..757578 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T243859 WP_003403386.1 NZ_CP096845:c754041-753604 [Mycobacterium tuberculosis variant bovis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T243859 NZ_OX637965:c114998-114693 [Escherichia coli O25b:H4-ST131]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT243859 WP_003403381.1 NZ_CP096845:c753494-753249 [Mycobacterium tuberculosis variant bovis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT243859 NZ_OX637965:c115218-115000 [Escherichia coli O25b:H4-ST131]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References