Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 692584..693230 Replicon chromosome
Accession NZ_CP096845
Organism Mycobacterium tuberculosis variant bovis strain Mb1101

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag M1777_RS03135 Protein ID WP_003403122.1
Coordinates 692584..692976 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag M1777_RS03140 Protein ID WP_003403125.1
Coordinates 692973..693230 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1777_RS03120 (M1777_03120) 688245..689772 + 1528 Protein_618 virulence factor Mce family protein -
M1777_RS03125 (M1777_03125) 689835..690977 + 1143 WP_003911253.1 virulence factor Mce family protein -
M1777_RS03130 (M1777_03130) 690982..692532 + 1551 WP_003403119.1 MlaD family protein -
M1777_RS03135 (M1777_03135) 692584..692976 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
M1777_RS03140 (M1777_03140) 692973..693230 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
M1777_RS03145 (M1777_03145) 693413..694648 - 1236 WP_003403128.1 ATP-binding protein -
M1777_RS03150 (M1777_03150) 694899..695312 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
M1777_RS03155 (M1777_03155) 695309..695545 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
M1777_RS03160 (M1777_03160) 695649..696155 - 507 WP_003900973.1 two component system sensor kinase HK1 -
M1777_RS03165 (M1777_03165) 696269..696799 - 531 WP_003917320.1 two component system sensor kinase HK2 -
M1777_RS03170 (M1777_03170) 696783..697478 - 696 WP_197044064.1 two-component system response regulator TcrA -
M1777_RS03175 (M1777_03175) 697601..697912 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T243851 WP_003403122.1 NZ_CP096845:c692976-692584 [Mycobacterium tuberculosis variant bovis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T243851 NZ_OX637964:c4148305-4148021 [Escherichia coli O25b:H4-ST131]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT243851 WP_003403125.1 NZ_CP096845:c693230-692973 [Mycobacterium tuberculosis variant bovis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT243851 NZ_OX637964:c4148541-4148308 [Escherichia coli O25b:H4-ST131]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References