Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2846454..2847085 Replicon chromosome
Accession NZ_CP096844
Organism Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag M1776_RS13365 Protein ID WP_003413174.1
Coordinates 2846708..2847085 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag M1776_RS13360 Protein ID WP_003413167.1
Coordinates 2846454..2846711 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1776_RS13325 (M1776_13330) 2841712..2842026 + 315 WP_009937839.1 hypothetical protein -
M1776_RS13330 (M1776_13335) 2842322..2843533 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
M1776_RS13335 (M1776_13340) 2843660..2844319 + 660 WP_273590156.1 LppA family lipoprotein -
M1776_RS13340 (M1776_13345) 2844316..2844975 + 660 WP_003900847.1 LppA family lipoprotein -
M1776_RS13345 (M1776_13350) 2844972..2845634 + 663 WP_003900848.1 LppA family lipoprotein -
M1776_RS13350 (M1776_13355) 2845631..2845909 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
M1776_RS13355 (M1776_13360) 2846002..2846415 + 414 WP_003413164.1 PIN domain nuclease -
M1776_RS13360 (M1776_13365) 2846454..2846711 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
M1776_RS13365 (M1776_13370) 2846708..2847085 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
M1776_RS13370 (M1776_13375) 2847101..2847475 - 375 WP_003413177.1 hypothetical protein -
M1776_RS13375 (M1776_13380) 2847575..2847970 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
M1776_RS13380 (M1776_13385) 2847967..2848212 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
M1776_RS13385 (M1776_13390) 2848623..2849042 - 420 WP_003413190.1 A24 family peptidase -
M1776_RS13390 (M1776_13395) 2849054..2849862 - 809 Protein_2643 shikimate dehydrogenase -
M1776_RS13395 (M1776_13400) 2849859..2851112 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
M1776_RS13400 (M1776_13405) 2851105..2851617 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T243831 WP_003413174.1 NZ_CP096844:2846708-2847085 [Mycobacterium tuberculosis variant bovis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T243831 NZ_OX637961:c4170182-4169805 [Escherichia coli O25b:H4-ST131]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT243831 WP_003413167.1 NZ_CP096844:2846454-2846711 [Mycobacterium tuberculosis variant bovis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT243831 NZ_OX637961:c4170639-4170271 [Escherichia coli O25b:H4-ST131]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References