Detailed information of TA system TA243813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1690114..1690730
• Replicon: chromosome
• Accession: NZ_CP096844
• Organism: Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TJ74
• Locus tag: M1776_RS07970
• Protein ID: WP_003407593.1
• Coordinates: 1690413..1690730 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TJ73
• Locus tag: M1776_RS07965
• Protein ID: WP_003900349.1
• Coordinates: 1690114..1690416 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1776_RS07955 (M1776_07965)	1686000..1687847	+	1848	WP_003407585.1	methylmalonyl-CoA mutase small subunit	-
M1776_RS07960 (M1776_07970)	1687848..1690100	+	2253	WP_003407587.1	methylmalonyl-CoA mutase	-
M1776_RS07965 (M1776_07975)	1690114..1690416	+	303	WP_003900349.1	type II toxin-antitoxin system antitoxin MazE4	Antitoxin
M1776_RS07970 (M1776_07980)	1690413..1690730	+	318	WP_003407593.1	type II toxin-antitoxin system toxin endoribonuclease MazF4	Toxin
M1776_RS07975 (M1776_07985)	1690727..1691731	+	1005	WP_003407596.1	methylmalonyl Co-A mutase-associated GTPase MeaB	-
M1776_RS07980 (M1776_07990)	1691784..1693074	+	1291	Protein_1573	serine hydrolase	-
M1776_RS07985 (M1776_07995)	1693147..1693764	-	618	WP_003407606.1	class I SAM-dependent methyltransferase	-
M1776_RS07990 (M1776_08000)	1693978..1694169	-	192	WP_003407609.1	dodecin family protein	-
M1776_RS07995 (M1776_08005)	1694251..1694649	+	399	WP_003900351.1	hypothetical protein	-
M1776_RS08000 (M1776_08010)	1694694..1695722	+	1029	WP_011799189.1	glycosyltransferase family 2 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11377.97 Da
• Isoelectric Point: 7.9950

>T243813 WP_003407593.1 NZ_CP096844:1690413-1690730 [Mycobacterium tuberculosis variant bovis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Nucleotide

• Length: 318 bp

>T243813 NZ_OX637961:291052-291579 [Escherichia coli O25b:H4-ST131]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11173.42 Da
• Isoelectric Point: 9.5564

>AT243813 WP_003900349.1 NZ_CP096844:1690114-1690416 [Mycobacterium tuberculosis variant bovis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Nucleotide

• Length: 303 bp

>AT243813 NZ_OX637961:290779-291045 [Escherichia coli O25b:H4-ST131]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 5XE3
• PDB: 5XE2

Antitoxin

• PDB: 5XE3
• AlphaFold DB: G0TJ73