Detailed information of TA system TA243809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1385513..1386201
• Replicon: chromosome
• Accession: NZ_CP096844
• Organism: Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: M1776_RS06625
• Protein ID: WP_003406304.1
• Coordinates: 1385770..1386201 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: M1776_RS06620
• Protein ID: WP_003406302.1
• Coordinates: 1385513..1385773 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1776_RS06600 (M1776_06600)	1381090..1381914	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
M1776_RS06605 (M1776_06605)	1381919..1383100	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
M1776_RS06610 (M1776_06610)	1383177..1384277	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
M1776_RS06615 (M1776_06615)	1384448..1385437	+	990	WP_003406301.1	malate dehydrogenase	-
M1776_RS06620 (M1776_06620)	1385513..1385773	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
M1776_RS06625 (M1776_06625)	1385770..1386201	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1776_RS06630 (M1776_06630)	1386224..1387910	-	1687	Protein_1308	PE family protein	-
M1776_RS06635 (M1776_06635)	1388090..1388950	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
M1776_RS06640 (M1776_06640)	1389031..1389861	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
M1776_RS06645 (M1776_06645)	1389918..1390211	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
M1776_RS06650 (M1776_06650)	1390208..1390477	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T243809 WP_003406304.1 NZ_CP096844:1385770-1386201 [Mycobacterium tuberculosis variant bovis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T243809 NZ_OX637960:c143803-143498 [Escherichia coli O25b:H4-ST131]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT243809 WP_003406302.1 NZ_CP096844:1385513-1385773 [Mycobacterium tuberculosis variant bovis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT243809 NZ_OX637960:c144023-143805 [Escherichia coli O25b:H4-ST131]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6