Detailed information of TA system TA243808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240710..1241278
• Replicon: chromosome
• Accession: NZ_CP096844
• Organism: Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: M1776_RS05950
• Protein ID: WP_003405865.1
• Coordinates: 1240904..1241278 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: M1776_RS05945
• Protein ID: WP_003405863.1
• Coordinates: 1240710..1240907 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1776_RS05925 (M1776_05930)	1236751..1237389	-	639	WP_011799158.1	lipid droplet-associated protein	-
M1776_RS05930 (M1776_05935)	1237479..1238486	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
M1776_RS05935 (M1776_05940)	1238503..1239486	-	984	WP_003898731.1	hypothetical protein	-
M1776_RS05940 (M1776_05945)	1239549..1240622	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
M1776_RS05945 (M1776_05950)	1240710..1240907	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1776_RS05950 (M1776_05955)	1240904..1241278	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1776_RS05955 (M1776_05960)	1241481..1242179	+	699	WP_010950494.1	hypothetical protein	-
M1776_RS05960 (M1776_05965)	1242297..1242482	+	186	WP_003901093.1	hypothetical protein	-
M1776_RS05965 (M1776_05970)	1242409..1242684	-	276	WP_003405867.1	hypothetical protein	-
M1776_RS05970 (M1776_05975)	1242927..1243250	+	324	WP_003405871.1	putative quinol monooxygenase	-
M1776_RS05975 (M1776_05980)	1243265..1243963	-	699	WP_031646953.1	hypothetical protein	-
M1776_RS05980 (M1776_05985)	1243997..1244206	+	210	WP_003911400.1	hypothetical protein	-
M1776_RS05985 (M1776_05990)	1244158..1244928	-	771	Protein_1179	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T243808 WP_003405865.1 NZ_CP096844:1240904-1241278 [Mycobacterium tuberculosis variant bovis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T243808 NZ_OX637959:c4382251-4382078 [Escherichia coli O25b:H4-ST131]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT243808 WP_003405863.1 NZ_CP096844:1240710-1240907 [Mycobacterium tuberculosis variant bovis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT243808 NZ_OX637959:c4382575-4382279 [Escherichia coli O25b:H4-ST131]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07