Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1024705..1025678 Replicon chromosome
Accession NZ_CP096844
Organism Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag M1776_RS04875 Protein ID WP_003404756.1
Coordinates 1025178..1025678 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID G0TFR4
Locus tag M1776_RS04870 Protein ID WP_003404754.1
Coordinates 1024705..1025181 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1776_RS04850 (M1776_04855) 1020552..1021823 - 1272 WP_003404747.1 PPE family protein -
M1776_RS04855 (M1776_04860) 1021838..1022137 - 300 WP_003404748.1 PE family protein -
M1776_RS04860 (M1776_04865) 1022581..1024362 + 1782 WP_003404749.1 BCCT family transporter -
M1776_RS04865 (M1776_04870) 1024362..1024616 + 255 WP_003898646.1 hypothetical protein -
M1776_RS04870 (M1776_04875) 1024705..1025181 + 477 WP_003404754.1 DUF1778 domain-containing protein Antitoxin
M1776_RS04875 (M1776_04880) 1025178..1025678 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
M1776_RS04885 (M1776_04890) 1025991..1027310 - 1320 WP_010950468.1 IS256-like element IS1554 family transposase -
M1776_RS04890 (M1776_04895) 1027598..1028179 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
M1776_RS04895 (M1776_04900) 1028179..1029831 + 1653 WP_003404761.1 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1024359..1070272 45913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T243805 WP_003404756.1 NZ_CP096844:1025178-1025678 [Mycobacterium tuberculosis variant bovis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T243805 NZ_OX637959:c3993431-3993117 [Escherichia coli O25b:H4-ST131]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17357.95 Da        Isoelectric Point: 11.2467

>AT243805 WP_003404754.1 NZ_CP096844:1024705-1025181 [Mycobacterium tuberculosis variant bovis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT243805 NZ_OX637959:c3993667-3993434 [Escherichia coli O25b:H4-ST131]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB G0TFR4

References