Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 717292..717941 Replicon chromosome
Accession NZ_CP096844
Organism Mycobacterium tuberculosis variant bovis strain Mb1855

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag M1776_RS03295 Protein ID WP_003403236.1
Coordinates 717546..717941 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag M1776_RS03290 Protein ID WP_003403235.1
Coordinates 717292..717546 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1776_RS03265 (M1776_03265) 712417..712500 + 84 Protein_647 galactose-1-phosphate uridylyltransferase -
M1776_RS03270 (M1776_03270) 712519..713601 + 1083 WP_031652152.1 galactose-1-phosphate uridylyltransferase -
M1776_RS03275 (M1776_03275) 713598..714689 + 1092 WP_003403225.1 galactokinase -
M1776_RS03280 (M1776_03280) 715084..716241 + 1158 WP_003903091.1 hypothetical protein -
M1776_RS03285 (M1776_03285) 716252..717199 + 948 WP_003403232.1 DUF732 domain-containing protein -
M1776_RS03290 (M1776_03290) 717292..717546 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
M1776_RS03295 (M1776_03295) 717546..717941 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
M1776_RS03300 (M1776_03300) 718035..718775 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
M1776_RS03305 (M1776_03305) 718907..719167 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M1776_RS03310 (M1776_03310) 719164..719571 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
M1776_RS03315 (M1776_03315) 719643..720794 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
M1776_RS03320 (M1776_03320) 720887..722614 - 1728 WP_010950423.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T243797 WP_003403236.1 NZ_CP096844:717546-717941 [Mycobacterium tuberculosis variant bovis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T243797 NZ_OX637959:2129929-2130031 [Escherichia coli O25b:H4-ST131]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT243797 WP_003403235.1 NZ_CP096844:717292-717546 [Mycobacterium tuberculosis variant bovis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT243797 NZ_OX637959:c2130069-2129801 [Escherichia coli O25b:H4-ST131]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References