Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2833063..2833700 Replicon chromosome
Accession NZ_CP096843
Organism Mycobacterium tuberculosis variant bovis strain Mb3602

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag M1781_RS13280 Protein ID WP_003413180.1
Coordinates 2833063..2833458 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag M1781_RS13285 Protein ID WP_003413183.1
Coordinates 2833455..2833700 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1781_RS13240 (M1781_13245) 2829148..2829807 + 660 WP_003900846.1 LppA family lipoprotein -
M1781_RS13245 (M1781_13250) 2829804..2830463 + 660 WP_003900847.1 LppA family lipoprotein -
M1781_RS13250 (M1781_13255) 2830460..2831122 + 663 WP_003900848.1 LppA family lipoprotein -
M1781_RS13255 (M1781_13260) 2831119..2831397 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
M1781_RS13260 (M1781_13265) 2831490..2831903 + 414 WP_003413164.1 PIN domain nuclease -
M1781_RS13265 (M1781_13270) 2831942..2832199 + 258 WP_003413167.1 CopG family transcriptional regulator -
M1781_RS13270 (M1781_13275) 2832196..2832573 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
M1781_RS13275 (M1781_13280) 2832589..2832963 - 375 WP_003413177.1 hypothetical protein -
M1781_RS13280 (M1781_13285) 2833063..2833458 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
M1781_RS13285 (M1781_13290) 2833455..2833700 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
M1781_RS13290 (M1781_13295) 2834111..2834530 - 420 WP_003413190.1 A24 family peptidase -
M1781_RS13295 (M1781_13300) 2834542..2835350 - 809 Protein_2624 shikimate dehydrogenase -
M1781_RS13300 (M1781_13305) 2835347..2836600 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
M1781_RS13305 (M1781_13310) 2836593..2837105 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T243773 WP_003413180.1 NZ_CP096843:c2833458-2833063 [Mycobacterium tuberculosis variant bovis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T243773 NZ_OX461105:c4520963-4520676 [Serratia proteamaculans]
ATGCTGGCCGTCAAATGGACTGACGAGGCAAAAACGGGTTTATATACCCTGATCGCTTTTATCGCGGAACAAAATCCAGT
TGCCGCTGAGTCGCTGATGCAGCGTATAGAAGAATCGGTCATTCCAGTAACTGAACATCCGTATTTGTTCCGAACCGGGC
GCGTAGCCGGAACTCGTGAAATAGTGGTACACCCAAATTATATAGTGATTTATCGCGTATTAGCGGAGCATATCGAGGTG
TTGAGTGTGCTTCATGCCCGTCAGGAATACCCTCGAACTTTGCCCTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT243773 WP_003413183.1 NZ_CP096843:c2833700-2833455 [Mycobacterium tuberculosis variant bovis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT243773 NZ_OX461105:c4521154-4520963 [Serratia proteamaculans]
ATGTCGACAAAATTGGACCCCATCGTCTCGGAGTTTGAAACTCAAGAGCAGGCTGACCGCTATGACCGTTGGTTTCGGAG
CCAGGTTGAAGCTGCAATCAATAGTAGCGCCCCTTTGATCCCTCATGATGAAGTCATGGCCGGTGCCCGTAAGATTATCG
AAGAAGCTAAAGCTAAAAGAAAATCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References