Detailed information of TA system TA024377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 5938394..5939020
• Replicon: chromosome
• Accession: NC_013406
• Organism: Paenibacillus sp. Y412MC10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W4ARZ2
• Locus tag: GYMC10_RS26020
• Protein ID: WP_006212388.1
• Coordinates: 5938394..5938744 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: GYMC10_RS26025
• Protein ID: WP_006212389.1
• Coordinates: 5938748..5939020 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYMC10_RS26000	5933715..5934065	+	351	WP_015737249.1	hypothetical protein	-
GYMC10_RS32045	5934146..5934277	+	132	WP_015737250.1	cortex morphogenetic protein CmpA	-
GYMC10_RS26005	5934376..5934846	-	471	WP_015737251.1	DinB family protein	-
GYMC10_RS26010	5934991..5935551	+	561	WP_015737252.1	ABATE domain-containing protein	-
GYMC10_RS26015	5935749..5937950	-	2202	WP_187293041.1	RNA-binding transcriptional accessory protein	-
GYMC10_RS26020	5938394..5938744	-	351	WP_006212388.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GYMC10_RS26025	5938748..5939020	-	273	WP_006212389.1	ribbon-helix-helix protein, CopG family	Antitoxin
GYMC10_RS26030	5939256..5940443	-	1188	WP_015737255.1	alanine racemase	-
GYMC10_RS26035	5940716..5941828	-	1113	WP_015737256.1	outer membrane lipoprotein carrier protein LolA	-
GYMC10_RS26040	5942092..5943420	-	1329	WP_015737257.1	MFS transporter	-
GYMC10_RS26045	5943482..5943982	-	501	WP_015737258.1	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12751.64 Da
• Isoelectric Point: 4.8265

>T24377 WP_006212388.1 NC_013406:c5938744-5938394 [Paenibacillus sp. Y412MC10]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAASHGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMSKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T24377 NC_013406:c5938744-5938394 [Paenibacillus sp. Y412MC10]
GTGATCGTTAAACGGGGCGACGTATTTTTTGCGGATTTATCACCTGTTGTCGGTTCTGAGCAAGGCGGCGTAAGGCCGGT
CCTTGTGATTCAGAACGATATCGGCAACCGGTTTAGTCCGACTGTGATTGTTGCAGCCATTACGGCACAGATTCAAAAAG
CCAAGCTGCCGACTCACGTCGAAATCGACGCGGCGTCGCATGGATTTGACCGGGATTCCGTCATTTTGCTAGAGCAGATC
CGGACGATTGATAAGCAGCGGCTAACCGACAAAATCACCCATCTTGATGATGAAATGATGAGCAAGGTGGATGATTCCCT
GCAAATAAGTCTGGGCTTGATTGATTTTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10500.04 Da
• Isoelectric Point: 6.4708

>AT24377 WP_006212389.1 NC_013406:c5939020-5938748 [Paenibacillus sp. Y412MC10]
MQNTKRIMISLPDHLLQEVDGIAQLENSNRSELIRQAMKLYLNERKKRYIRESMQRGYMEMAKINLTMASEAFHAEEDAD
NTLDRLVSGV

Nucleotide

• Length: 273 bp

>AT24377 NC_013406:c5939020-5938748 [Paenibacillus sp. Y412MC10]
ATGCAGAACACCAAACGAATCATGATCAGTTTACCGGATCATCTTTTGCAGGAAGTGGACGGGATCGCTCAGCTGGAAAA
TTCCAACCGCAGTGAGCTTATTAGGCAGGCCATGAAGTTGTACCTGAATGAACGGAAGAAACGTTACATTCGTGAATCCA
TGCAGCGGGGTTATATGGAAATGGCCAAAATCAACCTGACCATGGCTTCTGAGGCGTTTCACGCGGAGGAAGATGCAGAC
AACACTCTAGACCGCTTAGTAAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1S2EZN8