Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2300820..2301456 Replicon chromosome
Accession NZ_CP096843
Organism Mycobacterium tuberculosis variant bovis strain Mb3602

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag M1781_RS10730 Protein ID WP_003410654.1
Coordinates 2301046..2301456 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag M1781_RS10725 Protein ID WP_003410651.1
Coordinates 2300820..2301053 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1781_RS10710 (M1781_10720) 2296277..2296669 + 393 WP_229303073.1 metal ABC transporter permease -
M1781_RS10715 (M1781_10725) 2296670..2297074 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
M1781_RS10720 (M1781_10730) 2297158..2300742 - 3585 WP_010950652.1 cobaltochelatase subunit CobN -
M1781_RS10725 (M1781_10735) 2300820..2301053 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
M1781_RS10730 (M1781_10740) 2301046..2301456 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
M1781_RS10735 (M1781_10745) 2301440..2302531 + 1092 WP_003900454.1 precorrin-3B synthase -
M1781_RS10740 (M1781_10750) 2302541..2303167 + 627 WP_003410658.1 precorrin-8X methylmutase -
M1781_RS10745 (M1781_10755) 2303164..2304690 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
M1781_RS10750 (M1781_10760) 2304636..2305859 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T243766 WP_003410654.1 NZ_CP096843:2301046-2301456 [Mycobacterium tuberculosis variant bovis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T243766 NZ_OX461105:1671418-1671732 [Serratia proteamaculans]
ATGCAATTTACGGTATATGCAAATACAGGGAAAAGTACTGTTTACCCGCTGCTGCTTGATGTGACGAGCGATATCATCGG
GCAATTAAATCGTCGAGTCGTGATCCCGTTATTGCCCATTGAAAAATACCCAGGTAGCACTCGCCCGGAACGTTTGGTCC
CATTGGTCAGGCTGGTGGATGACAATGAGTACGCCGTTATGACTCATGAGATGGCCAGTATCCCCGTTCGTACCTTGGGC
GCTGAATTTTGCGATGCTACCCAATACCGAACCGAGGTCAAAGCCGCAATAGATTTTCTTCTCGACGGTATTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT243766 WP_003410651.1 NZ_CP096843:2300820-2301053 [Mycobacterium tuberculosis variant bovis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT243766 NZ_OX461105:1671179-1671415 [Serratia proteamaculans]
ATGCCAGTAAAACAACGAACGACGCAAAGCGTTACCATGACGGTAGAACGCAGCTTGTTGAGCCGTGCACGTGAGGCGGG
TATTAACCTCAGTGCTACGCTGAACGCTGCTTTGGATGCCGAATTGCGTCAGCATGAGATGAGAAAATGGAAGGAAGATA
ACCGAGAGGCTATTGAGGCTCTGAACCGTTTTCATGATGAACATGGCTGTTTCAGTGACGAATACAGGACGTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References