Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 88147..88748 Replicon plasmid pO111_2
Accession NC_013370
Organism Escherichia coli O111:H- str. 11128

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag ECO111_RS29540 Protein ID WP_001216034.1
Coordinates 88368..88748 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag ECO111_RS29535 Protein ID WP_001190712.1
Coordinates 88147..88368 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO111_RS29480 83864..84124 + 261 WP_000969528.1 hypothetical protein -
ECO111_RS29485 84124..84405 + 282 WP_000002123.1 ASCH domain-containing protein -
ECO111_RS29490 84429..84722 + 294 WP_000267997.1 hypothetical protein -
ECO111_RS29495 84729..85103 + 375 WP_000988652.1 hypothetical protein -
ECO111_RS29500 85085..85774 + 690 WP_000057456.1 hypothetical protein -
ECO111_RS29505 85758..86042 + 285 WP_001142389.1 hypothetical protein -
ECO111_RS29510 86042..86347 + 306 WP_000988220.1 hypothetical protein -
ECO111_RS29515 86344..86868 + 525 WP_000159954.1 toprim domain-containing protein -
ECO111_RS29520 86929..87144 - 216 WP_001048304.1 hypothetical protein -
ECO111_RS29525 87175..87510 - 336 WP_001133672.1 hypothetical protein -
ECO111_RS29530 87685..88074 + 390 WP_000506726.1 DNA repair protein -
ECO111_RS29535 88147..88368 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ECO111_RS29540 88368..88748 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ECO111_RS29545 88753..88932 + 180 WP_000113018.1 hypothetical protein -
ECO111_RS29550 88960..90003 + 1044 WP_000648823.1 DUF968 domain-containing protein -
ECO111_RS29555 90092..90544 + 453 WP_001448129.1 hypothetical protein -
ECO111_RS29560 90630..91823 + 1194 WP_000219603.1 terminase -
ECO111_RS29565 91823..93307 + 1485 WP_000124153.1 hypothetical protein -
ECO111_RS34460 93412..93642 + 231 Protein_108 ash family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..97897 97897


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T24375 WP_001216034.1 NC_013370:88368-88748 [Escherichia coli O111:H- str. 11128]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T24375 NC_013370:88368-88748 [Escherichia coli O111:H- str. 11128]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT24375 WP_001190712.1 NC_013370:88147-88368 [Escherichia coli O111:H- str. 11128]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT24375 NC_013370:88147-88368 [Escherichia coli O111:H- str. 11128]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References