Detailed information of TA system TA243749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1241137..1241705
• Replicon: chromosome
• Accession: NZ_CP096843
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3602

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: M1781_RS05960
• Protein ID: WP_003405865.1
• Coordinates: 1241331..1241705 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: M1781_RS05955
• Protein ID: WP_003405863.1
• Coordinates: 1241137..1241334 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1781_RS05935 (M1781_05935)	1237178..1237816	-	639	WP_003898730.1	lipid droplet-associated protein	-
M1781_RS05940 (M1781_05940)	1237906..1238913	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
M1781_RS05945 (M1781_05945)	1238930..1239913	-	984	WP_003898731.1	hypothetical protein	-
M1781_RS05950 (M1781_05950)	1239976..1241049	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
M1781_RS05955 (M1781_05955)	1241137..1241334	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1781_RS05960 (M1781_05960)	1241331..1241705	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1781_RS05965 (M1781_05965)	1241908..1242606	+	699	WP_010950494.1	hypothetical protein	-
M1781_RS05970 (M1781_05970)	1242724..1242909	+	186	WP_003901093.1	hypothetical protein	-
M1781_RS05975 (M1781_05975)	1242836..1243111	-	276	WP_003405867.1	hypothetical protein	-
M1781_RS05980 (M1781_05980)	1243354..1243677	+	324	WP_003405871.1	putative quinol monooxygenase	-
M1781_RS05985 (M1781_05985)	1243692..1244390	-	699	WP_031646953.1	hypothetical protein	-
M1781_RS05990 (M1781_05990)	1244424..1244633	+	210	WP_003911400.1	hypothetical protein	-
M1781_RS05995 (M1781_05995)	1244585..1245355	-	771	Protein_1181	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T243749 WP_003405865.1 NZ_CP096843:1241331-1241705 [Mycobacterium tuberculosis variant bovis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T243749 NZ_OX460973:500111-500461 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT243749 WP_003405863.1 NZ_CP096843:1241137-1241334 [Mycobacterium tuberculosis variant bovis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT243749 NZ_OX460973:499824-500105 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07