Detailed information of TA system TA243745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1015323..1015942
• Replicon: chromosome
• Accession: NZ_CP096843
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3602

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: M1781_RS04830
• Protein ID: WP_003404726.1
• Coordinates: 1015508..1015942 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: M1781_RS04825
• Protein ID: WP_003404724.1
• Coordinates: 1015323..1015502 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1781_RS04810 (M1781_04810)	1010796..1012376	+	1581	WP_011799139.1	serine hydrolase	-
M1781_RS04815 (M1781_04815)	1012373..1014766	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
M1781_RS04820 (M1781_04820)	1015039..1015197	+	159	WP_003404720.1	hypothetical protein	-
M1781_RS04825 (M1781_04825)	1015323..1015502	+	180	WP_003404724.1	antitoxin	Antitoxin
M1781_RS04830 (M1781_04830)	1015508..1015942	+	435	WP_003404726.1	SRPBCC family protein	Toxin
M1781_RS04835 (M1781_04835)	1016040..1016813	+	774	WP_003404735.1	VOC family protein	-
M1781_RS04840 (M1781_04840)	1016878..1017327	+	450	WP_003404738.1	hypothetical protein	-
M1781_RS04845 (M1781_04845)	1017462..1017692	-	231	WP_003898642.1	hypothetical protein	-
M1781_RS04850 (M1781_04850)	1017859..1019367	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
M1781_RS04855 (M1781_04855)	1019369..1020607	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T243745 WP_003404726.1 NZ_CP096843:1015508-1015942 [Mycobacterium tuberculosis variant bovis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T243745 NZ_OX460963:c4613545-4613204 [Hafnia paralvei]
ATGAACACCTCTCCTGCAACAAATCAGCGGGTAGCGAAGCCATGCCCGTCGCCTGTGGCCGTCTGGCAGATGTTACTGAC
CCGTCTACTTGCACAACACTATGGCCTGGCCCTGAGTGACACGCCATTCTGCGATGAAACAGTCATTCAGGAACATATCA
ACGCAGGCGTTACTCTTGCCGATGCCGTGAACTTTCTGGTGGAAAAATACGAACTCGTTCGCATCGATCGCAAAGGATTT
AGCTGGCAGGAACAATCGCCTTATCTGCGGGCTGTAGACATTTTGCGGGCGCGACAGGCAATGGGGTTGTTACGACAAGG
CCATAATCTTTCGACACGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT243745 WP_003404724.1 NZ_CP096843:1015323-1015502 [Mycobacterium tuberculosis variant bovis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT243745 NZ_OX460963:c4613925-4613581 [Hafnia paralvei]
ATGAAAAAAGACACAGCCAATGAAGTAGTGGATACGCTGCGATGGGGACTGAAATGCGACATCACACCACATTTCGGGGC
CCGTCTGGTGCAGGAAGGCAACCGCCTGCATTACCTCGCCGATCGGGCCAGCATAATCGGTCCGTTCAGCAAAGCTGATT
TACGCCATCTCAACCAGGCTTTCCCCTTGTTGATGAAGCAAATGGAGCTCATGCTCACCAGCGGAGAACTTAATTCCCGG
CACCAGCACTGCGTCACGCTGTATGCGAAAGGATTAACCTGTGAAGCCGATACGCTCGGCTCTTGTGGCTACGTCTATAT
CGCCATTTACCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: G0TFQ5