Detailed information of TA system TA243723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3142101..3142788
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: M1772_RS14955
• Protein ID: WP_003414624.1
• Coordinates: 3142345..3142788 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: M1772_RS14950
• Protein ID: WP_003414620.1
• Coordinates: 3142101..3142358 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS14930 (M1772_14920)	3137421..3138275	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
M1772_RS14935 (M1772_14925)	3138331..3139494	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
M1772_RS14940 (M1772_14930)	3139511..3140725	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
M1772_RS14945 (M1772_14935)	3140733..3141974	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
M1772_RS14950 (M1772_14940)	3142101..3142358	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
M1772_RS14955 (M1772_14945)	3142345..3142788	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1772_RS14960 (M1772_14950)	3142868..3143530	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
M1772_RS14965 (M1772_14955)	3143626..3143817	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
M1772_RS14970 (M1772_14960)	3144149..3145897	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
M1772_RS14975 (M1772_14965)	3145993..3146574	+	582	WP_003414644.1	fasciclin domain-containing protein	-
M1772_RS14980 (M1772_14970)	3146674..3146940	+	267	WP_031657293.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T243723 WP_003414624.1 NZ_CP096842:3142345-3142788 [Mycobacterium tuberculosis variant bovis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T243723 NZ_OX460940:c2066587-2066240 [Streptococcus agalactiae]
TTGGTATCTGATAATAAAACCCATAGCCTTATTATCCCTGAAACCGTACAAGAACAATTACAGGAGATAAAAAGCTATAT
TGAAACTACTTACTTTTCGGAGCAGGCAGGGGCTAACACCGTCAACAATATTCTTTACGGGCTAGAACGTCTTGAATTTT
TCCCAGAAGCAGGATTTAACGCAGATGACAGAGTAGGTGAAACTATTTACCCGCCACATAATACTCGTTGTATTGTTTTA
GGGGACTACCTAGCTTTTTATCACATTTTAGAAGACAGAAAAGCCGTTTTTGTATCTGATATTATCCATAGCAAACAAGA
CTATATCAAACTGTTTAAGAAAAAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT243723 WP_003414620.1 NZ_CP096842:3142101-3142358 [Mycobacterium tuberculosis variant bovis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT243723 NZ_OX460940:c2066864-2066577 [Streptococcus agalactiae]
ATGCACGCTTTAGAAAAAAATACGCAGGTCAACTTTAAAACCAACAGTGATTTACTAGAGAAAGCTAAAGCTATTATCGC
AGCCCAAAACCTTGACATGACAGCTAGCTTTAATTTGTTTTTAGAAAACATTGTACAAAATAAAGCCTTACCTTTTGAGA
CTGACACCGATAAAGAACGAGCTGAACTACTTGCAGGTTTACGAGCTGAAATTGCTAAAAGTTTTGACGATTTAGAGCAT
GGGCGGGTATACAACGCTAGTGAAGTGAGGGCTAACCTTGGTATCTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0