Detailed information of TA system TA243712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2823102..2823742
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: M1772_RS13225
• Protein ID: WP_003412970.1
• Coordinates: 2823102..2823521 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: M1772_RS13230
• Protein ID: WP_003412975.1
• Coordinates: 2823518..2823742 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS13195 (M1772_13185)	2818687..2819409	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
M1772_RS13200 (M1772_13190)	2819926..2820153	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
M1772_RS13205 (M1772_13195)	2820150..2820551	+	402	WP_003412963.1	PIN domain-containing protein	-
M1772_RS13210 (M1772_13200)	2820586..2821506	-	921	WP_011799273.1	restriction endonuclease	-
M1772_RS13215 (M1772_13205)	2821847..2822053	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
M1772_RS13220 (M1772_13210)	2822151..2823101	+	951	WP_162305733.1	ERCC4 domain-containing protein	-
M1772_RS13225 (M1772_13215)	2823102..2823521	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1772_RS13230 (M1772_13220)	2823518..2823742	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
M1772_RS13235 (M1772_13225)	2823773..2826616	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
M1772_RS13240 (M1772_13230)	2826688..2827089	-	402	WP_003412981.1	hypothetical protein	-
M1772_RS13245 (M1772_13235)	2827089..2827559	-	471	WP_003412985.1	transcription antitermination factor NusB	-
M1772_RS13250 (M1772_13240)	2827562..2828125	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T243712 WP_003412970.1 NZ_CP096842:c2823521-2823102 [Mycobacterium tuberculosis variant bovis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T243712 NZ_OX460931:c1957489-1957142 [Streptococcus agalactiae]
TTGGTATCTGATAATAAAACCCATAGCCTTATTATCCCTGAAACCGTACAAGAACAATTACAGGAGATAAAAAGCTATAT
TGAAACTACTTACTTTTCGGAGCAGGCAGGGGCTAACACCGTCAACAATATTCTTTACGGGCTAGAACGTCTTGAATTTT
TCCCAGAAGCAGGATTTAACGCAGATGACAGAGTAGGTGAAACTATTTACCCGCCACATAATACTCGTTGTATTGTTTTA
GGGGACTACCTAGCTTTTTATCACATTTTAGAAGACAGAAAAGCCGTTTTTGTATCTTATATTATCCATAGCAAACAAGA
CTATATCAAACTGTTTAAGAAAAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT243712 WP_003412975.1 NZ_CP096842:c2823742-2823518 [Mycobacterium tuberculosis variant bovis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT243712 NZ_OX460931:c1957766-1957479 [Streptococcus agalactiae]
ATGCACGCTTTAGAAAAAAATACGCAGGTCAACTTTAAAACCAACAGTGATTTACTAGAGAAAGCTAAAGCTATTATCGC
AGCCCAAAACCTTGACATGACAGCTAGCTTTAATTTGTTTTTAGAAAACATTGTACAAAATAAAGCCTTACCTTTTGAGA
CTGACACCGATAAAGAACGAGCTGAACTACTTGCAGGTTTACGAGCTGAAATTGCTAAAAGTTTTGACGATTTAGAGCAT
GGGCGGGTATACAACGCTAGTGAAGTGAGGGCTAACCTTGGTATCTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09