Detailed information of TA system TA243700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2190843..2191546
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: M1772_RS10180
• Protein ID: WP_003409778.1
• Coordinates: 2190843..2191172 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: M1772_RS10185
• Protein ID: WP_003409780.1
• Coordinates: 2191169..2191546 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS10160 (M1772_10155)	2187226..2188296	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
M1772_RS10165 (M1772_10160)	2188293..2188808	+	516	WP_003409718.1	flavin reductase family protein	-
M1772_RS10170 (M1772_10165)	2188805..2189866	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
M1772_RS10175 (M1772_10170)	2189863..2190633	+	771	WP_003409775.1	SDR family oxidoreductase	-
M1772_RS10180 (M1772_10175)	2190843..2191172	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1772_RS10185 (M1772_10180)	2191169..2191546	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
M1772_RS10190 (M1772_10185)	2191543..2192133	-	591	WP_003910477.1	SEC-C metal-binding domain-containing protein	-
M1772_RS10195 (M1772_10190)	2192188..2193552	+	1365	WP_003899094.1	HNH endonuclease signature motif containing protein	-
M1772_RS10200 (M1772_10195)	2193707..2194159	-	453	WP_010950636.1	lipoprotein	-
M1772_RS10205 (M1772_10200)	2194223..2194624	+	402	WP_003409869.1	hypothetical protein	-
M1772_RS10210 (M1772_10205)	2194617..2194799	-	183	WP_003409870.1	hypothetical protein	-
M1772_RS10215 (M1772_10210)	2194913..2195263	-	351	WP_003409871.1	hypothetical protein	-
M1772_RS10220 (M1772_10215)	2195274..2196176	-	903	WP_003409874.1	hypothetical protein	-
M1772_RS10225 (M1772_10220)	2196197..2196388	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T243700 WP_003409778.1 NZ_CP096842:c2191172-2190843 [Mycobacterium tuberculosis variant bovis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T243700 NZ_OX460906:c711194-710358 [Streptococcus agalactiae]
GTGAACAAAGCAAAATTAACGGCGCTCTGCCATAAAGTTTCAAAAGAAACAGGATTAACGTTTAATTCTGTGATGACCTA
CTACTTTCTGGAAATTATTCTGAAAAAATTGAGCCAAAGCGCTTATGCTCAAAATTACATTTTCAAAGGAGGTTTTCTGC
TCTCAAATGTCGTCGGGGTTGCTTCCCGTAGTACCGTTGACATTGACTTTCTCTTTAGTAAAATGAATCTATCAGAAGAG
ACCGTGCATCAGCAATTGGAGGATATCCTTGGAAAATCTGATGAAGATATTTCTTTCACTATTCAGTCGGTCACAGCAAT
TAAGGAAAGTGATGATTATGGAGGATATCGTGCAAGTATCCTTTGCCAGCTTGAGAACATCAGACAAGTCATCCACCTGG
ATATCGCAACTGGGGACATTGTCACTCCGCAGCCCATCACTTACGATTACAAGCCAATCTTCACATCAGAACAGTTTCCT
ATCATTGCCTACACGATTGAGACTATCTTAGCAGAGAAACTACAAACTATTTATTCTCGCAGCTTCTTGAATAGTCGAAG
CAAAGACTTCTATGACATCTATATCTTGTCAAAATTGAAGAAATCAGAAATTGATTCGCAACAACTTCTCACTGCTTGTC
AGCGTACCTTCGATTATCGAGGTACTGAATTAGACTTCGATAAATTCATCAATCTGCTAGAAACATTCAAAACAGATTCA
ACCCAAAACCATCAGTGGGAAAACTACTCTAAAAAGTATAGTTACACTAAAGGTATTACATTTAACGATGTTCTGACAGA
GATGACTGATTTGCTTGTAACTATCAAAAATCTCTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT243700 WP_003409780.1 NZ_CP096842:c2191546-2191169 [Mycobacterium tuberculosis variant bovis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT243700 NZ_OX460906:c711781-711191 [Streptococcus agalactiae]
ATGTCTAATAAAGAAACCGTTCTGAATTATCTCAAACAAAATAACGGTATCATTACATATAAAGACTGTAAAGAGCTTGG
GGTTCCAGCTGTCTATCTCTCACGACTTGAAAATGAAGAGAAAATATTCCGAGTGGACAAGGGCATCTATCTTTCTACCG
ATGGTGACTATGACGAGTACTACTTCTTCCAATACAGATTTTCAAGAGCTATTTACTCCTATGTTTCCGCTCTTTATCTT
CAAGGGTTCACGGATGAAATCCCTCAATATTTTGAAGTCACCGTACCAAGAGGGTATCGTTTCAATAATCCGCCTCAAAA
CCTTGCGGTTCATACCGTCCCAAAGGAAATTGTTGACCTTGGTATTATCTCTATAAAGACACCCTGGGGACAGACCGTTA
AGACCTACGACTTTGAGCGTATCATCTGTGACTTTATTCAAAATCGTAAAAAAATTGATAATGAACTTTTCGTCAAAACT
CTTAAGGCTTATGTCAACTATCCAAATAAAAACCTGACAAACCTTTACCAATATGCTGAAAAAATGAAAATTATCAATCA
AACAAAACAAACACTTGAGGTGCTAATGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8