Detailed information of TA system TA243697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1755068..1755681
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: M1772_RS08240
• Protein ID: WP_003407786.1
• Coordinates: 1755277..1755681 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: M1772_RS08235
• Protein ID: WP_009935474.1
• Coordinates: 1755068..1755271 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS08220 (M1772_08220)	1750332..1753235	+	2904	WP_003407779.1	MMPL family transporter	-
M1772_RS08225 (M1772_08225)	1753245..1753691	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
M1772_RS08230 (M1772_08230)	1753726..1755015	+	1290	WP_003407781.1	threonine ammonia-lyase	-
M1772_RS08235 (M1772_08235)	1755068..1755271	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1772_RS08240 (M1772_08240)	1755277..1755681	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
M1772_RS08245 (M1772_08245)	1755698..1757440	-	1743	WP_010950559.1	malto-oligosyltrehalose trehalohydrolase	-
M1772_RS08250 (M1772_08250)	1757433..1759730	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T243697 WP_003407786.1 NZ_CP096842:1755277-1755681 [Mycobacterium tuberculosis variant bovis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T243697 NZ_OX458335:c5908208-5907918 [Pseudomonas syringae pv. tomato]
ATGACTTATAGCCTCGAGTTTGATGCGCGAGCTCTGAAGGAATGGGGCAAATTAGGGGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCACCGCGCGTTGAAGCAAATCGTCTGCATGCGTTGCCCGACTGCTACAAAATCA
AACTGCGCAGCAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCTGTGGACAAG
CGTGAGCGAGATGAGGTTTACCGTAAGGCGGCTGATCGGTTGAGTGGGTAA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT243697 WP_009935474.1 NZ_CP096842:1755068-1755271 [Mycobacterium tuberculosis variant bovis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT243697 NZ_OX458335:c5908437-5908198 [Pseudomonas syringae pv. tomato]
ATGGCGTCTATTAATGTTCGTATCGACGACGATCTCAAAGCGCGTGCTTATCTCGAGCTTGAAAAGCTTGGCGTCACTCC
TTCGGAATTGCTGCGTCAAACCCTGCAGTATGTGGCAGAGCGCGGCCAGCTACCGTTCAAGACCGTACTGATGACCGAGG
AAGACGAAGCGTTGCTTGCAACAGTGCGCGAGCGCCTTGCGGCTCCGCAACGGGTCAAGGTTTCACTGGATGACTTATAG

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46