Detailed information of TA system TA243691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240509..1241077
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: M1772_RS05955
• Protein ID: WP_003405865.1
• Coordinates: 1240703..1241077 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: M1772_RS05950
• Protein ID: WP_003405863.1
• Coordinates: 1240509..1240706 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS05930 (M1772_05930)	1236550..1237188	-	639	WP_011799158.1	lipid droplet-associated protein	-
M1772_RS05935 (M1772_05935)	1237278..1238285	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
M1772_RS05940 (M1772_05940)	1238302..1239285	-	984	WP_003898731.1	hypothetical protein	-
M1772_RS05945 (M1772_05945)	1239348..1240421	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
M1772_RS05950 (M1772_05950)	1240509..1240706	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1772_RS05955 (M1772_05955)	1240703..1241077	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1772_RS05960 (M1772_05960)	1241280..1241978	+	699	WP_010950494.1	hypothetical protein	-
M1772_RS05965 (M1772_05965)	1242096..1242281	+	186	WP_003901093.1	hypothetical protein	-
M1772_RS05970 (M1772_05970)	1242208..1242483	-	276	WP_003405867.1	hypothetical protein	-
M1772_RS05975 (M1772_05975)	1242726..1243049	+	324	WP_003405871.1	putative quinol monooxygenase	-
M1772_RS05980 (M1772_05980)	1243064..1243762	-	699	WP_031646953.1	hypothetical protein	-
M1772_RS05985 (M1772_05985)	1243796..1244005	+	210	WP_003911400.1	hypothetical protein	-
M1772_RS05990 (M1772_05990)	1243957..1244727	-	771	Protein_1180	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T243691 WP_003405865.1 NZ_CP096842:1240703-1241077 [Mycobacterium tuberculosis variant bovis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T243691 NZ_OX458335:66048-66326 [Pseudomonas syringae pv. tomato]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGCTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGCGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AGCGGTCCCGAGGACGTAGAAATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT243691 WP_003405863.1 NZ_CP096842:1240509-1240706 [Mycobacterium tuberculosis variant bovis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT243691 NZ_OX458335:66336-66641 [Pseudomonas syringae pv. tomato]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGGGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07