Detailed information of TA system TA243687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1014980..1015599
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: M1772_RS04825
• Protein ID: WP_003404726.1
• Coordinates: 1015165..1015599 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: M1772_RS04820
• Protein ID: WP_003404724.1
• Coordinates: 1014980..1015159 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS04805 (M1772_04805)	1010453..1012033	+	1581	WP_011799139.1	serine hydrolase	-
M1772_RS04810 (M1772_04810)	1012030..1014423	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
M1772_RS04815 (M1772_04815)	1014696..1014854	+	159	WP_003404720.1	hypothetical protein	-
M1772_RS04820 (M1772_04820)	1014980..1015159	+	180	WP_003404724.1	antitoxin	Antitoxin
M1772_RS04825 (M1772_04825)	1015165..1015599	+	435	WP_003404726.1	SRPBCC family protein	Toxin
M1772_RS04830 (M1772_04830)	1015697..1016470	+	774	WP_003404735.1	VOC family protein	-
M1772_RS04835 (M1772_04835)	1016535..1016984	+	450	WP_003404738.1	hypothetical protein	-
M1772_RS04840 (M1772_04840)	1017119..1017349	-	231	WP_003898642.1	hypothetical protein	-
M1772_RS04845 (M1772_04845)	1017516..1019024	-	1509	WP_273590131.1	carotenoid oxygenase family protein	-
M1772_RS04850 (M1772_04850)	1019026..1020264	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T243687 WP_003404726.1 NZ_CP096842:1015165-1015599 [Mycobacterium tuberculosis variant bovis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T243687 NZ_OX458331:c1421419-1421225 [Anaerococcus sp. Marseille-Q7828]
ATGCCGATGACGTCAAAGCAAATGATTAAGTTCTTAAAGAAAAACGGATTTACTTATGTACCATCAGGAGATGGATCTCA
TAAGAAGTTCAAAAACTTTGAGACTGGTAAGGTTACAATAGTACCTGACCATGGGGGTAAGGATTTACCCAAGGGTACTG
AACACGTAATCTTGAAACAAGCGGGGCTAAAATAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT243687 WP_003404724.1 NZ_CP096842:1014980-1015159 [Mycobacterium tuberculosis variant bovis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT243687 NZ_OX458331:c1421188-1420757 [Anaerococcus sp. Marseille-Q7828]
ATGTTTGTTAATTATCCTGCTTTATTTTTAAAAGAAAAAGATAGTGATGCGTTTACTGTAGTTTTTCCAGATTTGCAGGG
ATGTGTTACTTATGGTGATAGCGTTAATGATGCTTTAAAAATGGCTCAGGATGCGTTAGGGGCATATCTGTTTGAATACT
ATACCAAGCCTAACGATATACCAAAGGCATCTAGTATAGATGATATTGAATTAAAGATAGATGAAGAAGATAAAGAATAC
TTTTTATATGAAGGTAGTTTTAAAAACTATGTTTCTTTAGATTTGACGGATTATGTAAAGAAATCTAGTATTAAAAATGT
TAAAAAAACTTTAACTATACCAAGTTATCTTAATGAAGCTGGGATTGAGAATAATATTAATTTCTCACTTTTATTGCAAG
AAGCTTTAAAAAAAGAATTGAAGATGATATAA

Structures

Antitoxin

• AlphaFold DB: G0TFQ5