Detailed information of TA system TA243686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 842867..843576
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: M1772_RS03985
• Protein ID: WP_183078491.1
• Coordinates: 843148..843576 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TEX4
• Locus tag: M1772_RS03980
• Protein ID: WP_003403834.1
• Coordinates: 842867..843124 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS03975 (M1772_03975)	840051..842776	+	2726	Protein_786	PE family protein	-
M1772_RS03980 (M1772_03980)	842867..843124	+	258	WP_003403834.1	ribbon-helix-helix protein, CopG family	Antitoxin
M1772_RS03985 (M1772_03985)	843148..843576	+	429	WP_183078491.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1772_RS03990 (M1772_03990)	843657..843794	-	138	WP_003403839.1	hypothetical protein	-
M1772_RS03995 (M1772_04000)	843953..844198	+	246	WP_003403841.1	hypothetical protein	-
M1772_RS04000 (M1772_04005)	844267..845151	-	885	WP_011799127.1	3-hydroxyisobutyrate dehydrogenase	-
M1772_RS04005 (M1772_04010)	845162..846334	-	1173	WP_010950438.1	acyl-CoA dehydrogenase family protein	-
M1772_RS04010 (M1772_04015)	846341..847873	-	1533	WP_069523346.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15829.24 Da
• Isoelectric Point: 7.5536

>T243686 WP_183078491.1 NZ_CP096842:843148-843576 [Mycobacterium tuberculosis variant bovis]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAETFAFVEAVTAQPHHL
PTNPGPRHLVLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL

Nucleotide

• Length: 429 bp

>T243686 NZ_OX458329:976944-977216 [Mobiluncus sp. Marseille-Q7826]
ATGCTAGAACTTAAGACAACAGCACAATTTCGTAAAGATTACAAACGCATCAAAAAACGCGGTTACAATCTTTCTTTGCT
CCAAGATGTGCTTGAGACACTCTGCGCAGAAAAGCCACTTGATGCAAACTACAAAGATCATGCACTGCTGGGTGCTTACA
AAGGCTTCCGAGAATGCCATATTCAACCTGATTGGCTTTTGATTTACACGACTGATAAAGACAAACTTATATTAGTTGCT
GCCCGCACAGGTAGTCACGCAGACTTGTTTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9114.28 Da
• Isoelectric Point: 10.6530

>AT243686 WP_003403834.1 NZ_CP096842:842867-843124 [Mycobacterium tuberculosis variant bovis]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Nucleotide

• Length: 258 bp

>AT243686 NZ_OX458329:976656-976940 [Mobiluncus sp. Marseille-Q7826]
ATGGCCAAAACCGCAAATATTAGTCTTCGTATAGAACCAGAAATAAAACACGAAGCGGATAAACTGTTCAGCAGCTTTGG
TATCTCTGTCACCGATGCCATTAATATTTTCCTGCGCACCTCCATTATGGAGGGCGGGTTTCCGTTCGCTATCAAACAAC
CCCGGTATAACGCTGAAACTGAAGCAGCTATAACTGAAGCACGAGACATTATATCTGGCAAAGTTCAGACAAATTCCTAT
AACTCTGCAACAGAACTCTTCAAATCCTTAGATACAGACGGCTAA

Structures

Antitoxin

• AlphaFold DB: G0TEX4