Detailed information of TA system TA243680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 717391..718040
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67241
• Locus tag: M1772_RS03295
• Protein ID: WP_003403236.1
• Coordinates: 717645..718040 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ34
• Locus tag: M1772_RS03290
• Protein ID: WP_003403235.1
• Coordinates: 717391..717645 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS03265 (M1772_03265)	712517..712600	+	84	Protein_647	galactose-1-phosphate uridylyltransferase	-
M1772_RS03270 (M1772_03270)	712619..713701	+	1083	WP_031652152.1	galactose-1-phosphate uridylyltransferase	-
M1772_RS03275 (M1772_03275)	713698..714789	+	1092	WP_003403225.1	galactokinase	-
M1772_RS03280 (M1772_03280)	715184..716341	+	1158	WP_003903091.1	hypothetical protein	-
M1772_RS03285 (M1772_03285)	716352..717298	+	947	Protein_651	DUF732 domain-containing protein	-
M1772_RS03290 (M1772_03290)	717391..717645	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	Antitoxin
M1772_RS03295 (M1772_03295)	717645..718040	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	Toxin
M1772_RS03300 (M1772_03300)	718134..718874	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
M1772_RS03305 (M1772_03305)	719006..719266	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
M1772_RS03310 (M1772_03310)	719263..719670	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	-
M1772_RS03315 (M1772_03315)	719742..720893	-	1152	WP_003403248.1	FIST N-terminal domain-containing protein	-
M1772_RS03320 (M1772_03320)	720986..722713	-	1728	WP_010950423.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14250.04 Da
• Isoelectric Point: 4.7475

>T243680 WP_003403236.1 NZ_CP096842:717645-718040 [Mycobacterium tuberculosis variant bovis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Nucleotide

• Length: 396 bp

>T243680 NZ_CP096842:717645-718040 [Mycobacterium tuberculosis variant bovis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9139.52 Da
• Isoelectric Point: 9.2702

>AT243680 WP_003403235.1 NZ_CP096842:717391-717645 [Mycobacterium tuberculosis variant bovis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Nucleotide

• Length: 255 bp

>AT243680 NZ_CP096842:717391-717645 [Mycobacterium tuberculosis variant bovis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• PDB: 4XGR
• PDB: 4XGQ

Antitoxin

• AlphaFold DB: A0A7U4BSC2