Detailed information of TA system TA243678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 704225..704868
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: M1772_RS03210
• Protein ID: WP_003403187.1
• Coordinates: 704467..704868 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: M1772_RS03205
• Protein ID: WP_003403184.1
• Coordinates: 704225..704470 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS03170 (M1772_03170)	699505..700035	-	531	WP_003917320.1	two component system sensor kinase HK2	-
M1772_RS03175 (M1772_03175)	700019..700714	-	696	WP_197044064.1	two-component system response regulator TcrA	-
M1772_RS03180 (M1772_03180)	700837..701148	+	312	WP_003403164.1	hypothetical protein	-
M1772_RS03185 (M1772_03185)	701220..702170	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
M1772_RS03190 (M1772_03190)	702411..702995	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
M1772_RS03195 (M1772_03195)	702997..703707	+	711	Protein_633	IS607 family element RNA-guided endonuclease TnpB	-
M1772_RS03200 (M1772_03200)	703710..704180	+	471	WP_003898523.1	hypothetical protein	-
M1772_RS03205 (M1772_03205)	704225..704470	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1772_RS03210 (M1772_03210)	704467..704868	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1772_RS03215 (M1772_03215)	704859..705038	+	180	Protein_637	hypothetical protein	-
M1772_RS03220 (M1772_03220)	705456..705653	-	198	WP_003403191.1	hypothetical protein	-
M1772_RS03225 (M1772_03225)	705733..706890	-	1158	WP_003403193.1	hypothetical protein	-
M1772_RS03230 (M1772_03230)	706942..707163	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
M1772_RS03235 (M1772_03235)	707305..707910	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T243678 WP_003403187.1 NZ_CP096842:704467-704868 [Mycobacterium tuberculosis variant bovis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T243678 NZ_OX442412:3432737-3433021 [Salmonella enterica subsp. enterica serovar Rissen]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTACCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT243678 WP_003403184.1 NZ_CP096842:704225-704470 [Mycobacterium tuberculosis variant bovis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT243678 NZ_OX442412:3432505-3432747 [Salmonella enterica subsp. enterica serovar Rissen]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91