Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5084690..5085187 Replicon chromosome
Accession NC_013364
Organism Escherichia coli O111:H- str. 11128

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECO111_RS26330 Protein ID WP_001173343.1
Coordinates 5085014..5085187 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECO111_RS26325 Protein ID WP_000398619.1
Coordinates 5084690..5084986 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO111_RS26300 5081421..5082140 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECO111_RS26305 5082137..5083768 - 1632 WP_001216487.1 sensor histidine kinase -
ECO111_RS26310 5083949..5084179 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
ECO111_RS26315 5084191..5084463 + 273 WP_000405647.1 N-acetyltransferase -
ECO111_RS26325 5084690..5084986 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECO111_RS26330 5085014..5085187 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECO111_RS26335 5085306..5086046 - 741 Protein_4963 lysine--tRNA ligase -
ECO111_RS26345 5086080..5087293 + 1214 WP_162829202.1 IS3 family transposase -
ECO111_RS33355 5087329..5087490 - 162 Protein_4965 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T24367 WP_001173343.1 NC_013364:5085014-5085187 [Escherichia coli O111:H- str. 11128]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T24367 NC_013364:5085014-5085187 [Escherichia coli O111:H- str. 11128]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT24367 WP_000398619.1 NC_013364:5084690-5084986 [Escherichia coli O111:H- str. 11128]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT24367 NC_013364:5084690-5084986 [Escherichia coli O111:H- str. 11128]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References