Detailed information of TA system TA243662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3089858..3090462
• Replicon: chromosome
• Accession: NZ_CP096841
• Organism: Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: M1774_RS14675
• Protein ID: WP_003414492.1
• Coordinates: 3089858..3090250 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: M1774_RS14680
• Protein ID: WP_003414495.1
• Coordinates: 3090247..3090462 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1774_RS14645 (M1774_14650)	3085008..3085796	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
M1774_RS14650 (M1774_14655)	3086130..3086675	-	546	WP_003904931.1	DUF1802 family protein	-
M1774_RS14655 (M1774_14660)	3086947..3087831	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
M1774_RS14660 (M1774_14665)	3087834..3088721	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
M1774_RS14665 (M1774_14670)	3089026..3089571	-	546	WP_009939112.1	DUF1802 family protein	-
M1774_RS14670 (M1774_14675)	3089568..3089837	-	270	WP_003912856.1	DUF2277 family protein	-
M1774_RS14675 (M1774_14680)	3089858..3090250	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1774_RS14680 (M1774_14685)	3090247..3090462	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
M1774_RS14685 (M1774_14690)	3090509..3091258	+	750	WP_003414497.1	enoyl-CoA hydratase	-
M1774_RS14690 (M1774_14695)	3091337..3092419	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
M1774_RS14695 (M1774_14700)	3092412..3093722	-	1311	WP_031702645.1	ABC transporter substrate-binding protein	-
M1774_RS14700 (M1774_14705)	3093725..3094552	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
M1774_RS14705 (M1774_14710)	3094549..3095460	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T243662 WP_003414492.1 NZ_CP096841:c3090250-3089858 [Mycobacterium tuberculosis variant bovis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T243662 NZ_OX419580:c2268298-2268047 [Bacillus subtilis]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGCAAACATGAAACGATTCTCATTATGGTT
TACCCATATCACATTCATTGGCTTATTCTTAATGTTTCAACTCATCAAAGATTACTTCAGCAGCGAAGGACAGGCGCTAA
TCAACACAATTTTTGTTGTCACATGTATCATTGCCATATTGTTATGGATCATCTATTGTGTATTCCTTAAACTAAGAAAC
AAGTCACACTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT243662 WP_003414495.1 NZ_CP096841:c3090462-3090247 [Mycobacterium tuberculosis variant bovis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT243662 NZ_OX419580:2268276-2268376 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8