243658

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3025390..3026069 Replicon chromosome
Accession NZ_CP096841
Organism Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag M1774_RS14305 Protein ID WP_003414059.1
Coordinates 3025390..3025806 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag M1774_RS14310 Protein ID WP_003414061.1
Coordinates 3025803..3026069 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1774_RS14285 (M1774_14285) 3021442..3022344 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
M1774_RS14290 (M1774_14290) 3022413..3023165 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
M1774_RS14295 (M1774_14300) 3023409..3023684 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
M1774_RS14300 (M1774_14305) 3023681..3025303 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
M1774_RS14305 (M1774_14310) 3025390..3025806 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
M1774_RS14310 (M1774_14315) 3025803..3026069 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M1774_RS14315 (M1774_14320) 3026095..3026490 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
M1774_RS14320 (M1774_14325) 3026487..3026756 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
M1774_RS14325 (M1774_14330) 3026766..3027860 - 1095 WP_003414068.1 restriction endonuclease subunit S -
M1774_RS14330 (M1774_14335) 3027857..3028276 - 420 Protein_2827 winged helix-turn-helix domain-containing protein -
M1774_RS14335 (M1774_14340) 3028275..3028349 + 75 Protein_2828 hypothetical protein -
M1774_RS14340 (M1774_14345) 3028350..3028829 - 480 WP_003414073.1 dihydrofolate reductase -
M1774_RS14345 (M1774_14350) 3028900..3029700 - 801 WP_003911953.1 thymidylate synthase -
M1774_RS14350 (M1774_14355) 3029856..3030593 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T243658 WP_003414059.1 NZ_CP096841:c3025806-3025390 [Mycobacterium tuberculosis variant bovis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T243658 NZ_OX419579:2269537-2269653 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT243658 WP_003414061.1 NZ_CP096841:c3026069-3025803 [Mycobacterium tuberculosis variant bovis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT243658 NZ_OX419579:c2269826-2269648 [Bacillus subtilis]
AATGATACAAATAATTATTTTTTGCATTTTGATTTGTAGAAATGCATAAAATAAAAAGACCAGGGTGCTACCAACACCCC
AGCTCTGTACAAAAAGCTGCCCATCAAAGGGCTTGCTCAGATGTATGTGACATAGTAGACCAACCCTTTAGGGTCGCAAT
CTCAAGGGGAGGTCTATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References