Detailed information of TA system TA243657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2896331..2897045
• Replicon: chromosome
• Accession: NZ_CP096841
• Organism: Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: M1774_RS13555
• Protein ID: WP_003413460.1
• Coordinates: 2896605..2897045 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: M1774_RS13550
• Protein ID: WP_003413456.1
• Coordinates: 2896331..2896618 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1774_RS13515 (M1774_13515)	2891754..2891999	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
M1774_RS13520 (M1774_13520)	2891996..2892400	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
M1774_RS13525 (M1774_13525)	2892617..2893237	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
M1774_RS13530 (M1774_13530)	2893248..2893742	+	495	WP_003413444.1	DUF2617 family protein	-
M1774_RS13535 (M1774_13535)	2893739..2894169	+	431	Protein_2672	DUF4247 domain-containing protein	-
M1774_RS13540 (M1774_13540)	2894194..2894652	+	459	WP_003413451.1	DUF350 domain-containing protein	-
M1774_RS13545 (M1774_13545)	2894649..2896220	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
M1774_RS13550 (M1774_13550)	2896331..2896618	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
M1774_RS13555 (M1774_13555)	2896605..2897045	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1774_RS13560 (M1774_13560)	2897066..2897821	-	756	WP_079367662.1	YebC/PmpR family DNA-binding transcriptional regulator	-
M1774_RS13565 (M1774_13565)	2897954..2898550	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
M1774_RS13570 (M1774_13570)	2898558..2899403	-	846	WP_010950749.1	acyl-CoA thioesterase II	-
M1774_RS13575 (M1774_13575)	2899432..2900331	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
M1774_RS13580 (M1774_13580)	2900459..2901133	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T243657 WP_003413460.1 NZ_CP096841:2896605-2897045 [Mycobacterium tuberculosis variant bovis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T243657 NZ_OX419579:c2217996-2217820 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGATAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT243657 WP_003413456.1 NZ_CP096841:2896331-2896618 [Mycobacterium tuberculosis variant bovis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT243657 NZ_OX419579:2217937-2218037 [Bacillus subtilis]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTATGATACCCACTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACACAATT

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8