Detailed information of TA system TA243655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2834864..2835495
• Replicon: chromosome
• Accession: NZ_CP096841
• Organism: Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: M1774_RS13280
• Protein ID: WP_003413174.1
• Coordinates: 2835118..2835495 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: M1774_RS13275
• Protein ID: WP_003413167.1
• Coordinates: 2834864..2835121 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1774_RS13240 (M1774_13240)	2830122..2830436	+	315	WP_009937839.1	hypothetical protein	-
M1774_RS13245 (M1774_13245)	2830732..2831943	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
M1774_RS13250 (M1774_13250)	2832070..2832729	+	660	WP_003900846.1	LppA family lipoprotein	-
M1774_RS13255 (M1774_13255)	2832726..2833385	+	660	WP_003900847.1	LppA family lipoprotein	-
M1774_RS13260 (M1774_13260)	2833382..2834044	+	663	WP_003900848.1	LppA family lipoprotein	-
M1774_RS13265 (M1774_13265)	2834041..2834319	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
M1774_RS13270 (M1774_13270)	2834412..2834825	+	414	WP_273620549.1	PIN domain nuclease	-
M1774_RS13275 (M1774_13275)	2834864..2835121	+	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
M1774_RS13280 (M1774_13280)	2835118..2835495	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1774_RS13285 (M1774_13285)	2835511..2835885	-	375	WP_003413177.1	hypothetical protein	-
M1774_RS13290 (M1774_13290)	2835985..2836380	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
M1774_RS13295 (M1774_13295)	2836377..2836622	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
M1774_RS13300 (M1774_13300)	2837033..2837452	-	420	WP_003413190.1	A24 family peptidase	-
M1774_RS13305 (M1774_13305)	2837464..2838272	-	809	Protein_2626	shikimate dehydrogenase	-
M1774_RS13310 (M1774_13310)	2838269..2839522	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
M1774_RS13315 (M1774_13315)	2839515..2840027	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T243655 WP_003413174.1 NZ_CP096841:2835118-2835495 [Mycobacterium tuberculosis variant bovis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T243655 NZ_OX419579:c2217801-2217550 [Bacillus subtilis]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGTAAACATGAAACGATTCTCACTATGGTT
TACCCATATCACATTCATTGGCTTATTCTTAATGTTTCAGCTCATCAAAGATTACTTCAGCAGCGAAGGAAAGGCACTAA
TCAACACAATTTTTGTAGTCACATGTATCATTGCCATACTGTTATGGATCATCTATTTTGTATTCCTTAAACTAAGAAAC
AAGTCACACTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT243655 WP_003413167.1 NZ_CP096841:2834864-2835121 [Mycobacterium tuberculosis variant bovis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT243655 NZ_OX419579:2217779-2217879 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5