Detailed information of TA system TA243653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2819869..2820509
• Replicon: chromosome
• Accession: NZ_CP096841
• Organism: Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: M1774_RS13180
• Protein ID: WP_003412970.1
• Coordinates: 2819869..2820288 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: M1774_RS13185
• Protein ID: WP_003412975.1
• Coordinates: 2820285..2820509 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1774_RS13150 (M1774_13150)	2815454..2816176	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
M1774_RS13155 (M1774_13155)	2816693..2816920	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
M1774_RS13160 (M1774_13160)	2816917..2817318	+	402	WP_003412963.1	PIN domain-containing protein	-
M1774_RS13165 (M1774_13165)	2817353..2818273	-	921	WP_003412965.1	restriction endonuclease	-
M1774_RS13170 (M1774_13170)	2818614..2818820	-	207	WP_234712436.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
M1774_RS13175 (M1774_13175)	2818918..2819868	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
M1774_RS13180 (M1774_13180)	2819869..2820288	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1774_RS13185 (M1774_13185)	2820285..2820509	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
M1774_RS13190 (M1774_13190)	2820540..2823383	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
M1774_RS13195 (M1774_13195)	2823455..2823856	-	402	WP_003412981.1	hypothetical protein	-
M1774_RS13200 (M1774_13200)	2823856..2824326	-	471	WP_003412985.1	transcription antitermination factor NusB	-
M1774_RS13205 (M1774_13205)	2824329..2824892	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T243653 WP_003412970.1 NZ_CP096841:c2820288-2819869 [Mycobacterium tuberculosis variant bovis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T243653 NZ_OX419579:517008-517358 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT243653 WP_003412975.1 NZ_CP096841:c2820509-2820285 [Mycobacterium tuberculosis variant bovis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT243653 NZ_OX419579:516722-517003 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09