Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2303483..2304119 Replicon chromosome
Accession NZ_CP096841
Organism Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag M1774_RS10735 Protein ID WP_003410654.1
Coordinates 2303709..2304119 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag M1774_RS10730 Protein ID WP_003410651.1
Coordinates 2303483..2303716 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1774_RS10715 (M1774_10725) 2298940..2299332 + 393 WP_229303073.1 metal ABC transporter permease -
M1774_RS10720 (M1774_10730) 2299333..2299737 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
M1774_RS10725 (M1774_10735) 2299821..2303405 - 3585 WP_010950652.1 cobaltochelatase subunit CobN -
M1774_RS10730 (M1774_10740) 2303483..2303716 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
M1774_RS10735 (M1774_10745) 2303709..2304119 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
M1774_RS10740 (M1774_10750) 2304103..2305194 + 1092 WP_003900454.1 precorrin-3B synthase -
M1774_RS10745 (M1774_10755) 2305204..2305830 + 627 WP_003410658.1 precorrin-8X methylmutase -
M1774_RS10750 (M1774_10760) 2305827..2307353 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
M1774_RS10755 (M1774_10765) 2307299..2308522 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T243649 WP_003410654.1 NZ_CP096841:2303709-2304119 [Mycobacterium tuberculosis variant bovis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T243649 NZ_OX419577:1867037-1867213 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTAACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGAGACAAGCAAAAAGAAAAAGCGTATACGTAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT243649 WP_003410651.1 NZ_CP096841:2303483-2303716 [Mycobacterium tuberculosis variant bovis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT243649 NZ_OX419577:c1867254-1867154 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTACGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References