Detailed information of TA system TA243647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2234339..2234965
• Replicon: chromosome
• Accession: NZ_CP096841
• Organism: Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: M1774_RS10435
• Protein ID: WP_003410075.1
• Coordinates: 2234567..2234965 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: M1774_RS10430
• Protein ID: WP_019283586.1
• Coordinates: 2234339..2234566 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1774_RS10420 (M1774_10430)	2232378..2232722	-	345	WP_003410065.1	ferredoxin family protein	-
M1774_RS10425 (M1774_10435)	2232911..2234080	-	1170	WP_003899126.1	ATP-binding protein	-
M1774_RS10430 (M1774_10440)	2234339..2234566	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1774_RS10435 (M1774_10445)	2234567..2234965	+	399	WP_003410075.1	PIN domain nuclease	Toxin
M1774_RS10440 (M1774_10450)	2235148..2235579	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
M1774_RS10445 (M1774_10455)	2235728..2236114	+	387	WP_003899128.1	DUF1398 domain-containing protein	-
M1774_RS10450 (M1774_10460)	2236551..2236730	-	180	Protein_2065	hypothetical protein	-
M1774_RS10455 (M1774_10465)	2236818..2237438	+	621	WP_003410086.1	IS110 family transposase	-
M1774_RS10460 (M1774_10470)	2237392..2237982	+	591	WP_003899131.1	IS110 family transposase	-
M1774_RS10465 (M1774_10475)	2238110..2239366	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T243647 WP_003410075.1 NZ_CP096841:2234567-2234965 [Mycobacterium tuberculosis variant bovis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T243647 NZ_OX419577:c1343780-1343034 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT243647 WP_019283586.1 NZ_CP096841:2234339-2234566 [Mycobacterium tuberculosis variant bovis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT243647 NZ_OX419577:c1343034-1342864 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• PDB: 4CHG