Detailed information of TA system TA243644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2201707..2202395
• Replicon: chromosome
• Accession: NZ_CP096841
• Organism: Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0A653
• Locus tag: M1774_RS10265
• Protein ID: WP_003409958.1
• Coordinates: 2201707..2202126 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: M1774_RS10270
• Protein ID: WP_003409968.1
• Coordinates: 2202135..2202395 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1774_RS10235 (M1774_10245)	2196714..2197019	+	306	Protein_2022	ABC transporter permease	-
M1774_RS10240 (M1774_10250)	2197015..2197095	+	81	Protein_2023	hypothetical protein	-
M1774_RS10245 (M1774_10255)	2197203..2198050	+	848	Protein_2024	class I SAM-dependent methyltransferase	-
M1774_RS10250 (M1774_10260)	2198013..2199458	-	1446	WP_003409946.1	APC family permease	-
M1774_RS10255 (M1774_10265)	2199637..2200323	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
M1774_RS10260 (M1774_10270)	2200514..2201482	-	969	WP_003409956.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
M1774_RS10265 (M1774_10275)	2201707..2202126	-	420	WP_003409958.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1774_RS10270 (M1774_10280)	2202135..2202395	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1774_RS10275 (M1774_10285)	2202538..2204214	+	1677	WP_010950639.1	PecA family PE domain-processing aspartic protease	-
M1774_RS10280 (M1774_10290)	2204202..2204855	-	654	WP_003409976.1	cutinase Cfp21	-
M1774_RS10285 (M1774_10295)	2204970..2205200	+	231	WP_003409980.1	DUF167 domain-containing protein	-
M1774_RS10290 (M1774_10300)	2205285..2206196	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
M1774_RS10295 (M1774_10305)	2206305..2206904	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T243644 WP_003409958.1 NZ_CP096841:c2202126-2201707 [Mycobacterium tuberculosis variant bovis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T243644 NZ_OX419576:517511-517861 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT243644 WP_003409968.1 NZ_CP096841:c2202395-2202135 [Mycobacterium tuberculosis variant bovis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT243644 NZ_OX419576:517225-517506 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A829C4H9

Antitoxin

• AlphaFold DB: A0A829C483