243641

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2183658..2184361 Replicon chromosome
Accession NZ_CP096841
Organism Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag M1774_RS10115 Protein ID WP_003409778.1
Coordinates 2183658..2183987 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag M1774_RS10120 Protein ID WP_003409780.1
Coordinates 2183984..2184361 (-) Length 126 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1774_RS10095 (M1774_10105) 2180041..2181111 + 1071 WP_003899091.1 epoxide hydrolase EphB -
M1774_RS10100 (M1774_10110) 2181108..2181623 + 516 WP_003409718.1 flavin reductase family protein -
M1774_RS10105 (M1774_10115) 2181620..2182681 + 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
M1774_RS10110 (M1774_10120) 2182678..2183448 + 771 WP_003409775.1 SDR family oxidoreductase -
M1774_RS10115 (M1774_10125) 2183658..2183987 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M1774_RS10120 (M1774_10130) 2183984..2184361 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
M1774_RS10125 (M1774_10135) 2184358..2184948 - 591 WP_003409784.1 SEC-C metal-binding domain-containing protein -
M1774_RS10130 (M1774_10140) 2185003..2186367 + 1365 WP_273620538.1 HNH endonuclease signature motif containing protein -
M1774_RS10135 (M1774_10145) 2186522..2186974 - 453 WP_010950636.1 lipoprotein -
M1774_RS10140 (M1774_10150) 2187038..2187439 + 402 WP_003409869.1 hypothetical protein -
M1774_RS10145 (M1774_10155) 2187432..2187614 - 183 WP_003409870.1 hypothetical protein -
M1774_RS10150 (M1774_10160) 2187728..2188078 - 351 WP_003409871.1 hypothetical protein -
M1774_RS10155 (M1774_10165) 2188089..2188990 - 902 Protein_2006 hypothetical protein -
M1774_RS10160 (M1774_10170) 2189011..2189202 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-103)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T243641 WP_003409778.1 NZ_CP096841:c2183987-2183658 [Mycobacterium tuberculosis variant bovis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T243641 NZ_OX419575:c1330522-1329776 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT243641 WP_003409780.1 NZ_CP096841:c2184361-2183984 [Mycobacterium tuberculosis variant bovis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT243641 NZ_OX419575:c1329776-1329606 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TLK7


Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References