Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3620460..3621114 Replicon chromosome
Accession NC_013364
Organism Escherichia coli O111:H- str. 11128

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECO111_RS19010 Protein ID WP_000244777.1
Coordinates 3620460..3620867 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECO111_RS19015 Protein ID WP_000354046.1
Coordinates 3620848..3621114 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO111_RS18990 3616417..3618150 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
ECO111_RS18995 3618156..3618866 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECO111_RS19000 3618891..3619787 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECO111_RS19005 3619899..3620420 + 522 WP_001055874.1 flavodoxin FldB -
ECO111_RS19010 3620460..3620867 - 408 WP_000244777.1 toxin CptA Toxin
ECO111_RS19015 3620848..3621114 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECO111_RS19020 3621357..3622337 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
ECO111_RS19025 3622533..3623192 - 660 WP_000250274.1 hemolysin III family protein -
ECO111_RS19030 3623356..3623667 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
ECO111_RS19035 3623712..3625145 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
ECO111_RS19040 3625202..3625945 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T24364 WP_000244777.1 NC_013364:c3620867-3620460 [Escherichia coli O111:H- str. 11128]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T24364 NC_013364:c3620867-3620460 [Escherichia coli O111:H- str. 11128]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT24364 WP_000354046.1 NC_013364:c3621114-3620848 [Escherichia coli O111:H- str. 11128]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT24364 NC_013364:c3621114-3620848 [Escherichia coli O111:H- str. 11128]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References