Detailed information of TA system TA243633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1387395..1388083
• Replicon: chromosome
• Accession: NZ_CP096841
• Organism: Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: M1774_RS06640
• Protein ID: WP_003406304.1
• Coordinates: 1387652..1388083 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: M1774_RS06635
• Protein ID: WP_003406302.1
• Coordinates: 1387395..1387655 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1774_RS06615 (M1774_06615)	1382972..1383796	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
M1774_RS06620 (M1774_06620)	1383801..1384982	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
M1774_RS06625 (M1774_06625)	1385059..1386159	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
M1774_RS06630 (M1774_06630)	1386330..1387319	+	990	WP_003406301.1	malate dehydrogenase	-
M1774_RS06635 (M1774_06635)	1387395..1387655	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
M1774_RS06640 (M1774_06640)	1387652..1388083	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1774_RS06645 (M1774_06645)	1388106..1389792	-	1687	Protein_1311	PE family protein	-
M1774_RS06650 (M1774_06650)	1389972..1390832	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
M1774_RS06655 (M1774_06655)	1390913..1391743	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
M1774_RS06660 (M1774_06660)	1391800..1392093	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
M1774_RS06665 (M1774_06665)	1392090..1392359	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T243633 WP_003406304.1 NZ_CP096841:1387652-1388083 [Mycobacterium tuberculosis variant bovis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T243633 NZ_OX419570:2621161-2621340 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT243633 WP_003406302.1 NZ_CP096841:1387395-1387655 [Mycobacterium tuberculosis variant bovis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT243633 NZ_OX419570:c2621486-2621264 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6