Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3478700..3479283 Replicon chromosome
Accession NC_013364
Organism Escherichia coli O111:H- str. 11128

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag ECO111_RS18345 Protein ID WP_000254745.1
Coordinates 3478700..3479035 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag ECO111_RS18350 Protein ID WP_000581937.1
Coordinates 3479035..3479283 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO111_RS18330 3474587..3475885 - 1299 WP_000036723.1 phosphopyruvate hydratase -
ECO111_RS18335 3475973..3477610 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
ECO111_RS18340 3477838..3478629 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
ECO111_RS18345 3478700..3479035 - 336 WP_000254745.1 endoribonuclease MazF Toxin
ECO111_RS18350 3479035..3479283 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ECO111_RS18355 3479361..3481595 - 2235 WP_000226815.1 GTP pyrophosphokinase -
ECO111_RS18360 3481643..3482943 - 1301 Protein_3473 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T24363 WP_000254745.1 NC_013364:c3479035-3478700 [Escherichia coli O111:H- str. 11128]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T24363 NC_013364:c3479035-3478700 [Escherichia coli O111:H- str. 11128]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT24363 WP_000581937.1 NC_013364:c3479283-3479035 [Escherichia coli O111:H- str. 11128]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT24363 NC_013364:c3479283-3479035 [Escherichia coli O111:H- str. 11128]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References