Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1026282..1027255 Replicon chromosome
Accession NZ_CP096841
Organism Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag M1774_RS04890 Protein ID WP_003404756.1
Coordinates 1026755..1027255 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID G0TFR4
Locus tag M1774_RS04885 Protein ID WP_003404754.1
Coordinates 1026282..1026758 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1774_RS04865 (M1774_04865) 1022129..1023400 - 1272 WP_003404747.1 PPE family protein -
M1774_RS04870 (M1774_04870) 1023415..1023714 - 300 WP_003404748.1 PE family protein -
M1774_RS04875 (M1774_04875) 1024158..1025939 + 1782 WP_003404749.1 BCCT family transporter -
M1774_RS04880 (M1774_04880) 1025939..1026193 + 255 WP_003898646.1 hypothetical protein -
M1774_RS04885 (M1774_04885) 1026282..1026758 + 477 WP_003404754.1 DUF1778 domain-containing protein Antitoxin
M1774_RS04890 (M1774_04890) 1026755..1027255 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
M1774_RS04900 (M1774_04900) 1027568..1028887 - 1320 WP_010950468.1 IS256-like element IS1554 family transposase -
M1774_RS04905 (M1774_04905) 1029175..1029756 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
M1774_RS04910 (M1774_04910) 1029756..1031408 + 1653 WP_003404761.1 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1025936..1071848 45912


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T243629 WP_003404756.1 NZ_CP096841:1026755-1027255 [Mycobacterium tuberculosis variant bovis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T243629 NZ_OX419569:2723554-2723733 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17357.95 Da        Isoelectric Point: 11.2467

>AT243629 WP_003404754.1 NZ_CP096841:1026282-1026758 [Mycobacterium tuberculosis variant bovis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT243629 NZ_OX419569:c2723879-2723657 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB G0TFR4

References