Detailed information of TA system TA243618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 699493..700139
• Replicon: chromosome
• Accession: NZ_CP096841
• Organism: Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: M1774_RS03165
• Protein ID: WP_003403137.1
• Coordinates: 699493..699906 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: M1774_RS03170
• Protein ID: WP_003403139.1
• Coordinates: 699903..700139 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1774_RS03145 (M1774_03145)	695576..697126	+	1551	WP_003403119.1	MlaD family protein	-
M1774_RS03150 (M1774_03150)	697178..697570	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
M1774_RS03155 (M1774_03155)	697567..697824	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
M1774_RS03160 (M1774_03160)	698007..699242	-	1236	WP_003403128.1	ATP-binding protein	-
M1774_RS03165 (M1774_03165)	699493..699906	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1774_RS03170 (M1774_03170)	699903..700139	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
M1774_RS03175 (M1774_03175)	700243..700749	-	507	WP_003900973.1	two component system sensor kinase HK1	-
M1774_RS03180 (M1774_03180)	700863..701393	-	531	WP_003917320.1	two component system sensor kinase HK2	-
M1774_RS03185 (M1774_03185)	701377..702072	-	696	WP_197044064.1	two-component system response regulator TcrA	-
M1774_RS03190 (M1774_03190)	702195..702506	+	312	WP_003403164.1	hypothetical protein	-
M1774_RS03195 (M1774_03195)	702578..703528	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
M1774_RS03200 (M1774_03200)	703769..704353	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
M1774_RS03205 (M1774_03205)	704355..705065	+	711	Protein_635	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T243618 WP_003403137.1 NZ_CP096841:c699906-699493 [Mycobacterium tuberculosis variant bovis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T243618 NZ_OX419567:518540-518890 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT243618 WP_003403139.1 NZ_CP096841:c700139-699903 [Mycobacterium tuberculosis variant bovis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT243618 NZ_OX419567:518254-518535 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89