Detailed information of TA system TA024361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3157163..3157788
• Replicon: chromosome
• Accession: NC_013364
• Organism: Escherichia coli O111:H- str. 11128

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ECO111_RS16695
• Protein ID: WP_000911330.1
• Coordinates: 3157163..3157561 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: ECO111_RS16700
• Protein ID: WP_000450524.1
• Coordinates: 3157561..3157788 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO111_RS16675	3153041..3153241	+	201	WP_000383836.1	YpfN family protein	-
ECO111_RS16680	3153351..3154049	-	699	WP_000679812.1	esterase	-
ECO111_RS16685	3154123..3156138	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
ECO111_RS16690	3156153..3157016	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
ECO111_RS16695	3157163..3157561	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ECO111_RS16700	3157561..3157788	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
ECO111_RS16705	3157942..3158655	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
ECO111_RS16715	3158868..3159902	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
ECO111_RS16720	3159919..3160797	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
ECO111_RS16725	3160943..3161527	+	585	WP_000176194.1	glycine cleavage system transcriptional repressor	-
ECO111_RS16730	3161527..3161997	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T24361 WP_000911330.1 NC_013364:c3157561-3157163 [Escherichia coli O111:H- str. 11128]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T24361 NC_013364:c3157561-3157163 [Escherichia coli O111:H- str. 11128]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT24361 WP_000450524.1 NC_013364:c3157788-3157561 [Escherichia coli O111:H- str. 11128]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT24361 NC_013364:c3157788-3157561 [Escherichia coli O111:H- str. 11128]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33