Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3067923..3068493 Replicon chromosome
Accession NZ_CP096840
Organism Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag M1780_RS14565 Protein ID WP_003414166.1
Coordinates 3067923..3068279 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag M1780_RS14570 Protein ID WP_003901465.1
Coordinates 3068263..3068493 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1780_RS14545 (M1780_14545) 3063375..3065063 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
M1780_RS14550 (M1780_14550) 3065067..3065393 - 327 WP_003414157.1 hypothetical protein -
M1780_RS14555 (M1780_14555) 3065566..3066153 + 588 WP_003914429.1 DUF3558 family protein -
M1780_RS14560 (M1780_14560) 3066172..3067821 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
M1780_RS14565 (M1780_14565) 3067923..3068279 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
M1780_RS14570 (M1780_14570) 3068263..3068493 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
M1780_RS14575 (M1780_14575) 3068536..3069579 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
M1780_RS14580 (M1780_14580) 3069578..3070045 + 468 WP_003414177.1 DUF1778 domain-containing protein -
M1780_RS14585 (M1780_14585) 3070221..3070475 - 255 WP_003917684.1 hypothetical protein -
M1780_RS14590 (M1780_14590) 3070623..3071027 + 405 WP_003414181.1 hypothetical protein -
M1780_RS14595 (M1780_14595) 3071024..3071215 + 192 WP_003414184.1 hypothetical protein -
M1780_RS14600 (M1780_14600) 3071432..3071692 + 261 Protein_2881 transposase -
M1780_RS14605 (M1780_14605) 3072802..3073059 + 258 WP_003899489.1 hypothetical protein -
M1780_RS14610 (M1780_14610) 3073164..3073475 + 312 WP_010950777.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T243601 WP_003414166.1 NZ_CP096840:c3068279-3067923 [Mycobacterium tuberculosis variant bovis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T243601 NZ_OX419557:2262271-2262387 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT243601 WP_003901465.1 NZ_CP096840:c3068493-3068263 [Mycobacterium tuberculosis variant bovis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT243601 NZ_OX419557:c2262560-2262382 [Bacillus subtilis]
AATGATACAAATAATTATTTTTTGCATTTTGATTTGTAGAAATGCATAAAATAAAAAGACCAGGGTGCTACCAACACCCC
AGCTCTGTACAAAAAGCTGCCCATCAAAGGGCTTGCTCAGATGTATGTGACATAGTAGACCAACCCTTTAGGGTCGCAAT
CTCAAGGGGAGGTCTATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References