Detailed information of TA system TA243598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2898780..2899494
• Replicon: chromosome
• Accession: NZ_CP096840
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: M1780_RS13585
• Protein ID: WP_003413460.1
• Coordinates: 2899054..2899494 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: M1780_RS13580
• Protein ID: WP_003413456.1
• Coordinates: 2898780..2899067 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1780_RS13545 (M1780_13540)	2894202..2894447	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
M1780_RS13550 (M1780_13545)	2894444..2894848	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
M1780_RS13555 (M1780_13550)	2895065..2895685	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
M1780_RS13560 (M1780_13555)	2895696..2896190	+	495	WP_003413444.1	DUF2617 family protein	-
M1780_RS13565 (M1780_13560)	2896187..2896618	+	432	WP_003413445.1	DUF4247 domain-containing protein	-
M1780_RS13570 (M1780_13565)	2896643..2897101	+	459	WP_003413451.1	DUF350 domain-containing protein	-
M1780_RS13575 (M1780_13570)	2897098..2898669	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
M1780_RS13580 (M1780_13575)	2898780..2899067	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
M1780_RS13585 (M1780_13580)	2899054..2899494	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1780_RS13590 (M1780_13585)	2899515..2900270	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
M1780_RS13595 (M1780_13590)	2900403..2900999	-	597	WP_273610390.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
M1780_RS13600 (M1780_13595)	2901007..2901852	-	846	WP_010950749.1	acyl-CoA thioesterase II	-
M1780_RS13605 (M1780_13600)	2901881..2902780	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
M1780_RS13610 (M1780_13605)	2902908..2903582	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T243598 WP_003413460.1 NZ_CP096840:2899054-2899494 [Mycobacterium tuberculosis variant bovis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T243598 NZ_OX419557:c2211528-2211277 [Bacillus subtilis]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGTAAACATGAAACGATTCTCACTATGGTT
TACCCATATTACATTCATTGGCTTATTCTTAATGTTTCAACTCATCAAAGATTACTTCAGCAGCGAAGGAAAGGCACTAA
TCAACACAATATTTGTAGTCACATGTATCATTGCCATACTGTTATGGATCATCTATTTTGTATTCCTTAAACTAAGAAAC
AAGTCACACTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT243598 WP_003413456.1 NZ_CP096840:2898780-2899067 [Mycobacterium tuberculosis variant bovis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT243598 NZ_OX419557:2211506-2211606 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8